                           ˿µϣ20170802գ

17.7.29-8.1ûи
17.08.02, ӡȲܷ°
17.08.02, ӡԪΪΰ첻ȥˡ
17.08.02, FMĿӽѧ172:˭ȥԪ
17.08.02, ٱǧ˼ƻùоɹ걨йȻѧ
17.08.02, ƽԪΪʲôԻΪ
17.08.02, ıµĻ༭𣿡
17.08.02, Alex Berezowšѧ֮Ӧ֮
17.08.02, תе֪
17.08.02, 곷 ӱƴѧ鼰س
17.08.02, Ȼ-＼ۣǸ˵ʱ
17.08.02, ѡɣӷĵڿ15
17.08.02, غϼ1792017.7.11-20

˿(www.xys.org)(xys10.dxiong.com)(xys.ebookdiy.com)(fangzhouzi.me)

                 Ȳܷ°

                    ӡ

ͳУеǲͿȡֵǣһ˵Ŀ
϶彡෴áȾ𺦽ǳٺȿ
ΪҸ档һȻʰ֢оΪܵ°ǰ
ƽйؿȵıõо𽥶෴أһ
ΪϣرڲĹйĵλޱȳߣ
ƺǶƣϼңϣҶɴ౻Ϊסҽ
ѧԲıҲŨȤʮоıõгǧ
ƪоȵĶöࡣЩоҪŪȲǷܹԤ֢
Ѫܼ򲡵ԲоģǺȲ방֢Ĺϵ

ҽѧоֲƺȷж潡ĳɷ֣ҪһЩ
ʣҪһжӵĿ250ˮ2.5̲裬
620880ˮʣдԼ3040%ǶӡͬĲ
ղͬӵĺҲ̶̲ͬȺܵͣ˶⵽
ƻӵĺߡĶӺϵͣΪ˸
ĶʣغͲأǺҲǿͨΪ
Ԥ֢ãܷĻҪ鹦Щ
ʵӡغͲܹưϸʵҲ
ͲеĶֹܹദٳ֤ⷽǺǿġ

ܲ˾˵ȲȷеĿѧأܡʵͶʵ
һܹƹ㵽ϡоȲ˻ĹϵҪͨ
ѧ飬ȽϺȲȺͲȲȺĻ֢ķա֤ⷽݺ
еĻìܡЩоֺȲܹͻٰǰ᳦ٰ
ѳͷΰķգ͵òࡣҲЩоֺȲ貢ܽͻ
֢աǰܹвѧǺֲܴڵģԵóۡ
вѧһٴ顣оȲ방֢ϵٴ
鲻࣬ҪоȲǷܽĳ방֢йصʶĺ
һʶ8-ǻգDNA˺ģ
Һ8-ǻպȽϸߣԤʾŰ֢ձȽϸߡһٴ
У124Ϊ͸׺ͽӴùиΰ߷յʵ
ֳ飬ÿֱڷ500˺1000̲ʣ൱
24̲裩һڷοΪաº󣬷̲
8-ǻյĺֻͣǶһ롣ǲ֪
8-ǻյĺǷľζŽ˰֢աҲе
ٴ鷢ֺȲ貢ܽͰ֢ʶĺеٴ鷢֣
пǻǰĻߣڷ̲ȡͰķաֻ
С͵ٴ飬ҪнһĴٴܵóۡ

ȻкܶʵͶʵ鶼Ȳܹвѧ
ٴĽȴһ¡ֲһԿʹõʵϲͬйء
ͬĲҶࣨ͵̲衢ȫ͵ĺ軹ǲַ͵裿
Ҷ췽÷ʽòеĳɷַܴı仯Ӷõ
һ¡ңͨȲĶʺǺܵ͵ģҪ
ʵͶʵʹõ12ҲԽΪʲô˺Ȳ
ЧʵͶʵôԡĿǰ޷鿿Ȳ


Ȳ跽ʽǿ°Ӣо֣Ⱥ̵Ȳ˵
ʳķǺ²˵ⱻΪӢŮʳʸߵԭ
ˮʳµģʵ޹ءϲȲĻ
ҪżݳĲһٺȡ˻˵ҸɴȲϻ
ȫֲʺǳͣ˶û̫洦
ŶʾǲҶʵĻҪˮݲȡ

Ҷ򴢴淽ʽҲ°աҴС˺Ȳϰߣ
ÿ춼Ҫȹ裬ҪǺϼҲ裬Ҳ裬żҲ
̲衢裬һֲǴȵģǾն衣ΪȲ
ߣųƺܺáܹնҺһɷùζǳڽ
Ŀǡնղͬں󷢽Ͳ裬ͳǰë
ڳˮȻͣ˵ŵʱԽԽԶƷ
ԽáִЩĽǴͬС졣ն軹ν
֣֮պųȻ״̬³»ģû׶ѷʹ
ͬڴʱֻ̱ͣȽϻҪʱȽϳѡ
ַ͡£жҲնȾ
ùءءŻ¶صȸءЩǻùأ
ǿҵ°֮һôںն裬ǲǻӻ֢ķأ

ֵǵĵȻֻһһֱдԺն°ҵר
Ҳһֱ֮ĹýϡҥһҶԺʿ˵
նǲẬлùصģΪֻжŻն
кǲģ˵úնرܣܹ˷ֱ桢ѡ
ĺûƵġи̨ĲҶڣеն費ʲô
ģͨͨдùǣֻҪ80ˮĽݣùΪ㣬
ԴҲõġ̨Ҷ͵ǲǵĺն
ᱻùȾǵùضΣ80ȵˮɱùȴ
ùʧȥԣùҪ280϶ȵĸ²ʧȥԡ
ũҵѧնѧԺоԱʱʱط֤նмⲻ
ùءܼ⵽ùؾ͹ˣȾĲǡ

ն漰޴ľ棬Щط˵Ƕšе
Ϊն绤ˣδͷֻûпѧϲ뵱Ȼ
һӿʳƷȫĿѧʣϷƪ£
աΪʲôõնĻùȾоɣһ
Ʒն輸ֻܳùȾڶնÿ
٣Լֻ510ˡùزˮݲʱ򣬶
ȫҺȲʱҲѲҶԽȥ

ЩòʵáնƷʺûǸƷ֡
ζʱģûлùûйϵնǰ
ûùصĲⶨô֪ƷնͲùأⲻ
նƭ˵ĻԶնᱻΪƷĲ
ǡǡȾùصķոߡնÿ510ˣǶ٣
ȡغĸߵͣûκݣô˵ٵΪ
ǣοͨȵģٳɶࡣùزˮڳݹ
лΪϸԭùͬܽб˺ȽȥҲ˾
ν

ЩΪն绤˵ǶǲΪƾģؼҪ֤ݡû֤
ݱгն躬лùأеġ2010мԤ
оԱ˹гϵ70նƷȫܼùأ
8ݻùصĺй׼涨Ļùֵ5΢/ǧ
ˣͬʱնƷзغŻ¶أ63Ż
¶صĺ˱׼涨ֵ1/ǧˣ2012꣬ϲѧһʳ
Ʒ˶ʿоظ˹ݼĵоҲ͹ݼо
һ£ϲгɼ60ն裬ȫܼùأ7ݳꡣ
Ҳȫ˷غŻ¶أ41Ż¶سꡣ

ɼгնձ麬лùغأеĺǳߡ
ôںȲʱЩûпܱȥΣأܡ2012갲
ʡɽҽԺڿƱһһÿ10ն裬
һº󣬷ùж¼Ը𺦡ߺȵնлù
صĺ30΢/ǧˡÿź10ն裬λѧʡ
νٵķΧҲǰѲҶԽȥģǺȵģĻùص
Ѹߵ˼жοǵ뵼µ𺦣˵
ʹÿֻ10ն裬ʹùزˮնеĻùܹͬ
˺Ե˺׷֣Ե˺°ε
ˣ׷֡Ϊ׷֣뵱ȻؽдҲҪģν
աǺ˵αա

ǰҿչа˸Ⱦ棬
񶼺ܷŭֿն躬ܶ˺أ
ѵгʵضвôʳϰߡҵĸϼңϲ
ɣͬ°ʰ߷ܾ͸ʳϰйء
°ʳƷһһ㶼ֻҪ˽գζͽ
֮Ȩ⡣ϰ˺ն裬ҲΪҵĿվŵò
ҺˣնǴɲ˷ŭֻ
նʱҪ֪°յģҪն費°ﷴ
õ˵ΪбĲʺҵĿζҲõĻ
ùغأûбҪðպնˡ

2017.6.12.

ѧ硷2017.7

(XYS20170802)

˿(www.xys.org)(xys10.dxiong.com)(xys.ebookdiy.com)(fangzhouzi.me)

                   ԪΪΰ첻ȥ

                           ӡ

ǰԪһҺųרתʳƷ豹ڴԪ
˵Ǵȥ豹εְ˳˾йɷݼְ
̶̵ļ仰ÿ䶼ǲôţӦǴԪ
ʣʡȻдõģ˼Ҳ޷˵Լ
ΪתˡӴ漯š⵽˱ˡ񶾵
ˮΪǵҵǣʹְˡ

뵱ԪվһţϺý⵽ڶɡ¶
ɡ¶ߵазתʿžǴԪν
Ӵ漯šԪǰһֱ˵漯šɽ˾
ȻҲûл֯͡ϵϣ˵ķ˿Ϊһˡ
ɡ¶ԪʿУҪӴöǸתûκιϵ
ͨýˣԪװ֪ˡǲ漯ŵ
ˣ۵ɡȨ˵һ⵽ɡ¶˵
ˮˣһǡ񶾵ˮءؼɡ¶ǲиоݣ
УǾûУǲ˵Ǳˮ

ɴԪҪĵ㣺һƷ۸ߣףν
ʳƷƷϢм٣ٹ棻乩ӦĴͱ
ʼ첿żϸ񣬶ýϱ˵ĻѧĴð
ϰѹեĴͣġɶɣǰͶ
ʯͷֽǷԱʺǷѱִŲ⡣ĵɣ
ʵݵģûмԪ豹ʵԵķǻ
һЩ¼һЩƷɾĳЩϢɼҲǳɡ
¶öԵģô˵ǱˮأԪ˵ǵҵǣ
ˣָ֪ʲôɡ¶ʱûǣˡŶ
ˣڳĹɶѱġʯͷֽˣЩǲǾǴԪ
ˣ

Ԫýɷã˼˵⵽Ĩڣ˵Լܵв
ƣԼҵַˣ˴ŷţȥѧУݽ
˺ѧ᣻˱ʾѾյɱָڱйֱ
˾в˱ҵˣ赭д˵ǿЦ¶͹ȥˡ֪
˭ô󵨣ȻвȫЭίԱҹֻôЭίԱ
ȫϡȻûм˭ԴԪвΪ
еĹܵвҲǲܵġһʱҲܵв
ֻ빫ҵסַųҪɱҡ˴ܵ
ҵʦ˵绰˵ɱˣŵǸϿ챨ҵ
飬ͨΪвص棬ȥ飬ȥˣǾǵ
ˣ˲鵽ǲ׷ŹģôúŪģǱĹ
ԴԪ˵ְҵ˼˵һǿЦͷŹˣ̫
硣ǰԪ˵ӴְΪȥأ
Һû˵в

ԪȻ˵ⳡУҪѪսף˸Ѫ
׼ˣ˼һв˳꣬ôܽѪս
أҪѪսףӦüֲһеذ꿪ȥǰ
Ʒ۸ߣ˵һǼۣеѪս׵
˵ѵҵǣҪڷͳһҲֻ
˵вû˵вѣǳһ˵ģ
ѳϣʲô˼أʳƷĻʳƷ
ҪҪʿ״ʲôأ

Ԫǰýɷ˵һţǴһط
˼ΪԱÿ˽ǧԪǲôԸ⽻ô
˰Ȼֵûɡǲж˳ĻԱǶ
ŴԪͷڶԴԪμġڴԪ˳ˣ
ʹԪûκιϵˣЩ˻ԸԱǵĻ
Ĺɶ㡰ʯͷֽʱԸԺҲԱʣǷ
ָǰյֵĻ³ڵĻԱҪάȨԪ
ܣʯͷֽԱάȨʱ򣬺ų2ڴԷѵĴԪ
ȫܵġ

2017.7.27

(XYS20170802)

˿(www.xys.org)(xys10.dxiong.com)(xys.ebookdiy.com)(fangzhouzi.me)

FMĿӽѧ172:˭ȥԪ

https://fm.qq.com/luobo/radio?_wv=4097&aid=rd001USq1949X1mp&showid=rd000OYuqk3CjmXW&sourceInfo=adpos%3D20034

(XYS20170802)

˿(www.xys.org)(xys10.dxiong.com)(xys.ebookdiy.com)(fangzhouzi.me)

ٱǧ˼ƻùоɹ걨йȻѧ

ڹоɹ걨ȻѧȽ̲
Ȼ໥»Ƶоɻͨύ8ƪ
УƪоɹȫԼս˹ѧȡõģ

1.Induction of APOBEC3G ubiquitination and degradation by an HIV-1 
Vif-Cul5-SCF complex. / Science / Yu X, Yu Y, Liu B, Luo K, Kong W, 
Mao P, Yu XF.

2.T-cell differentiation factor CBF-? regulates HIV-1 Vif-mediated 
evasion of host restriction. / Nature / Zhang W, Du J, Evans SL, Yu Y, 
Yu XF.

3.Selective assembly of HIV-1 Vif-Cul5-ElonginB-ElonginC E3 
ubiquitin ligase complex through a novel SOCS box and upstream 
cysteines./ Genes & development / Yu Y, Xiao Z, Ehrlich ES, Yu X, Yu XF.

4.Primate lentiviral virion infectivity factors are substrate 
receptors that assemble with cullin 5-E3 ligase through a HCCH motif 
to suppress APOBEC3G. / Proceedings of the National Academy of 
Sciences of the United States of America / Luo K, Xiao Z, Ehrlich E, 
Yu Y, Liu B, Shu Z, Yu XF.

ЩԼս˹ѧȫְڼȡõĿгɹ
ڹڶѧְʵʵʱ伫٣Ϊڹȡ棬
ЩʱϹڴѧԵһɵλھȻһ
걨Ȼѧ

(XYS20170802)

˿(www.xys.org)(xys10.dxiong.com)(xys.ebookdiy.com)(fangzhouzi.me)

ԪΪʲôԻΪ

ߣƽ

ͿΪ㲻սԻΪʱȴģ
ٶ˿ȥ֣Ԫɵġ

Ԫ1˵м˵Ҫ2ڷڷѣתۺ͵顣
˵ûˣǶҲӦǡ

ҴսѾȻ2ڣҪôܣսҪôܣ
ع2ڵ˭սɴȫʤûع⣬˵û2ڣǻԡ

Ԫ2˵˸ŷš˺ٱѧݽ
ʱᡣյ˰ɱָ

ҴпţԪ᲻ع𣿺ģԪ᲻
عڰɱԪ˵򱱾йֱˣҲҵǸˣ
˵ǿЦģ¾͹ȥˡһСƷйֲܰı
ɱСƷһݣ˵Ĳ滰

Ԫ3˵˳豹ΪӴ漯źⱨ

Ҵĸ漯ţǾͲ滰Ī漯ŵı
ָǣָ豹ƵġתСסΥ涨Ϊ
ûתСףС׶ǷתģʳƷܲż豹
ת͡ÿﺬ14ܼұ׼ǲü150
ýع豹ôԪ֪ȣ5000Ԫ3ԱԱ
ũƷͬƷ5۸񣬱1ֻ300Ԫ

ԪΪʲô˵ѣйءѧΪҲ˵
ѣǾб˸˸˵ѡ˸ı־ҷΣ
Ϊģгԡ 

(XYS20170802)

˿(www.xys.org)(xys10.dxiong.com)(xys.ebookdiy.com)(fangzhouzi.me)

ıµĻ༭

ߣ
Դйѧ

201782գȻ-＼ƣغŶ20165
2շڸڿģĳǺŶģһ䶯һ
ʱ鳤õ¼ڿƽϢˣǿѧ̽ѰµĻ༭Ľ
ͣϢ

༭ϴ

죬༭ܡʵҲһϷչ
ġ

;ʮһΪпָøZFNпָø
пָ׺ͺøɲ֣ǰ߸ʶDNAУ
DNAжϣϸDNA޸ܣʵֶĿĻΡ

ZFNWord༭һĿĻ޸ģ֮ǰͬ
Դ鼼˻óЧʣӶΪһ༭
ʮɧ

ZFNܶԻκλ޸ģѰЧӦϸߣҲ
ڽ϶ķ༭ҹɱҷʱ2009꣬ѧֿ
һƵĻ༭ϵת¼ЧӦӺø
TALENǵڶ༭üֻǽʶDNAеԪΪ
TALE ףȻTALENȴZFN׵ö࣬ҲͬڽϸߵѰ
ЧӦ

2012꣬ݴѧУĿѧҿһȫµĻ༭
2015걻ѧ־Ϊʮȿѧͻ֮׵CRISPR/Cas9
Ҳǵ༭ҲҪĻ༭

ͨRNAѰĿУɱͣҿԾȷ
ѰЧӦҲƣCRISPR/Cas9Ѹٱõѧоĸ
ڶ̶ʱڣг΢ֲͶ
ϸ̥Ŵ죬չֳ˾ϲǰ

ӸCRISPR/Cas9ƺǲϴǿѧ
ԡ

NgAgo-gDNA

һЩѧһѰµĻ༭ߣͻмרơ

20165£ԺӱƼѧĺ격ʿйȫ
ľϲоŶڹʶѧ־Ȼ-＼Ϸһƪģ
һμϸзһֺøûȵ£
ʶУ༭ΪһȫµĻ༭ߡ
NgAgo-gDNA

һͱýΪŵɹϺԲ֪ѧ
ûи߼ְƣҲûѧıһʱ𼫴䶯

ŵý屨걾Ҳ˻ڶ棬ӱ
ʡЭϯΪҪǣӱʡΪ˱ֿ֧Ƽµĳ⣬
2000Ԫʵҽ衣


º󣬹NgAgo-gDNA޷ظƼ
ʿӷ֮󱱾ѧڵǰ֪ͦѧҲ
ӱƼѧ¡

1116գ21ڡϸ־Ϸģ
ʵҾʾ޷ظĵĽ

1128գԺ׶ѧ¹ѧ÷оԺ10
λѧڡȻ＼Ϸ£ͬʾδܼ
NgAgo-gDNAĻ༭Ч

֮Ȼ＼־뺫Ŷӻͨ2017
1µ׹յ201782գȻ-＼ƣ
غŶ201652շڸڿġ

ȻϵأǸ¼ҲйһЩ׶ˣһ
гɹΨӢۣһƪӰӵģƺʲôˣ
Щֿ֧Ƽ¸Ըѩ̿ȱѧ
ƣ¼࣬ھȻû֯Ч飬û
ŵĵ

µĻ༭Խ

ȻNgAgo-gDNAսCRISPR/Cas9л༭ϣԽԽ
ãѰµĻ༭ȻǿѧŬһҪ

2016915գйϾѧоԱڹ֪ڿ
ѧϱһڽṹĺøĻ༭¼
ǿʵDNAеİиһоɹѧ
ûNgAgo-gDNAӦȳʵӦЧдһ
졣

ȻҪҵȫµĻ༭߾£Ժܶѧڶ
иĽͼʹˡЧ㡣

2015ףCRISPR/Cas9֮һĻѧŷ岩ʿڡϸ
־ҵһµøCas9ΪCas9ȣø
ֻҪһRNAӣСڽϸ༭ЧãCas9
λòͬṩѡ

20165º12£ŷŶڡϸ͡Ȼ＼
ƪ£ֱøľṹԼͬʱ༭

һCRISPR/Cas9ĹѫѧҰŦҮ
Emmanuelle CharpentierʿŶ4ҲڡȻ־Ϸģʾ
˸øDNA⣬ܶCRISPR RNAΣǸû༭
ϵͳʵֶ༭Ҫԭ

Ÿ˼༭Խ֮Ул༭רƽ
͹ԣ̽ѰµĻ༭ߺ͸Ľл༭ڱУڲ
ĽؽָЧĻ༭

(XYS20170802)

˿(www.xys.org)(xys10.dxiong.com)(xys.ebookdiy.com)(fangzhouzi.me)

šѧ֮Ӧ֮

Alex Berezowר
룺պ

һ̽;ŵȳ档ǹ͹
δȡ˽ѡ

ͬˣǵĽȡǶԿѧȷʶˣǱ뽫
֮սȥЩѧʱͽȵӰ졣
ʮժҵ顶ѧСƤ顷

Ϳ˥ϲƷǻΪʲôǵ˥ϣ
˲ٹĸָļ衣һ˵ǣ
ѹ³´лһָƷʱϸϽ
ֹܷ̣ٴδܹ֤ʵᡱѷͨ
ȷӪ˶һŴ󲿷ִƷܾƭǮġ

޻ѧĲƷκζһֻѧȷȷʵʵκζ
Ŀȵˮŵıֽûʲô޻ѧͨ
ĻѧʾЩԻѧͶѧһϲͨĻ
Ŵڣʹ֮ʳͻɹ

ȥÿ춼ԽȥһЩжʡдΧȻ
ڡ׺飬ﺬм׻ǵ³´лҲ
һЩжʡϢǣܹشЩءȥ
ȥߡࡱΪĸѾΡ

֮͡ԳΪͣΪǺȡֲġ
ΪӪľֲͬȡͿܺ
ûȻʳƷͿȡԴֲǿ
㡣Ҫģİΰٲ

ʲ͡лʵ˺١в˶ⲿ
кЩзкУʲ;һָȡ
ʳʲͺӳǣܿΪȥʳж̼ˮ
;ǵȣоá

ȻĸáҽҩлʳƷ˶ȵȣһжԴԡȻ
ĸá㷺񻰡Ҳѧƿںš컨̣
٣˪βߺͶЫȻģ츣ҩȴǡ

лʳƷлʳƷһ޴ƭܹ֡ƣ12Ԫ
עÿ㽶ӽӺóԣҲûиӪ߸
ڻֲлʳƷҲʹóʮֳɹùû
ʹáлũҵǳЧ޷ιȫ硣

ū޼󡣿ū޼ҲƤʿࡱʵǱЩǮȳʶ
ľӢǳʱ֡һū޼ǽģʲô
ħǿһʱ䣬ƶ³˺Ͳά
˶ͬĹڵʳƷΪˡ

ʳûκȥĻûƷ֭ࡣ
ǰʳðϷΪɱΣյģһЩģ
΢ѧưʳеӪɷ֡ʵϣ
Ľĸ߷ա

ơĳЩʳһϡһֳʳΪûʲ
ôʳǳʳָЧɷ֣أڶЧ
ҪֱעѪΪڷһλŮ
顣

Щʱǵͻø͸п
ѧ֪ʶʵɵ磬ǵ˽ѧͰʱˡ

Alex Berezow, ѧͽίԱҽѧԱӵ΢ѧ
ʿѧλǡͶ߶»Աժ顶
ѧСƤ顷

(XYS20170802)

˿(www.xys.org)(xys10.dxiong.com)(xys.ebookdiy.com)(fangzhouzi.me)

תе֪

ߣ
廪ѧ봫ѧԺڣʿʦ

תųΪý屨罻͹ճеһ
⡣ԶԣǰؿƼת＼
ڹȥʮ䲻һЩ¼ʹùڶ֮ԳĹעۡ

ܶڹ㷺۵Ļһתһȷͳһ
ıʵڲͬﾳ£̺źܲȷĺӽǣ漰⣬
ӼƷӲҵܡӿѧ⵽ֵ⡢ʳƷȫ
ȫӹó׵Ұȫһ㡣Щڡתһͳ
ĶֶĹעǶȣйۺгͬʱ֣
۱Ҫ߲ԵĽил¸˵ĶԻ
Чչ

򵥶ԣת漰ɷΪƷ/Ӧú
⡣Ƕת֪Ҳ漰档Ҫ̸̸
ͲƷ֪

ת

תִ＼Ҫɲ֡˼壬ת漰
Ĵô򵽵ʲô

˵ǺӡҪǾ˫ṹǺἴ
DNAӡкضŴϢƬΡDNAÿϸеϸе
ʡͨνȾɫ壬ϸлҪ壬DNA͵
ɡϸṹ͹ܵĻλΪ˵ÿһٺ֯
ɴϸɡÿһϸж˰һ˼ŴϢ
DNAӡ-ϸ-DNA-ĹϵĿǿ
״ĻŴλ

תͨ˵Ẉ̂ҪָDNAӼ򹤳̣
ָǵԸԻΡȣӶظıŴ
״ļ˹ιĻ뵽У״
ĿŴı䣬һ̾νת򡣴ӱԣתһָ
Ŵ״ļֻڷˮƽϽеŴ
״ı漰йתĹУҲһҪȡģ
ǶתԵ˼

ʵԴǵȴδֹͣŴ
ȥǧũķʽҪǶȻͻѡ
ãͨȻķʽʳõĴ󲿷
˹ϹǾĸ̶ݱġת봫ͳ
һеģʶͨŴ

ȻڻתƵķΧЧϣת봫ͳּҪ
һǴͳһֻͬڸлתƣת
ͻԵϵƣʵּֿĻתƣǴͳ
ӽѡһˮƽϽеģ飬
תƵǴĻ򣬲׼ȷضĳвκѡ񣬶Ժ
״ֵԤԽϲתתƵһǾȷ
򣬹翹桢ȣֿ׼ȷԤڡͳӽֺ
תֵ𣬿Ȼʵ;ȷʵ֮ͬȡ

תֱص㣬ܵһЩԭ
Ϊѧ1973귢DNAת＼
Ҳ漴ʼԷӦÿܶ彡ͻĲ
оԺNIH֯ѧҺ淽ƶ1976귢
DNAо׼򡷣ΪϵһDNẠҲΪ͹
֯ƶﰲȫ׼ľοı10Уѧ߰칫
1986귢＼Эܡʽȷ＼·
аԭ򣬼ƷԭǿעղƷת򷽷ѧ
ԭ򣬼ֻдڿɾѧ֤ʵķʱſɶתƷӦ
жĹܿأʵʵͬԭ򣬼תƷвƷʵͬ
ԶмܡÿɹҿѧᣨNSFũҵUSDA
ʳƷҩƷ֣FDAEPA͹оԺNIH
ִС

ѧ뼼߰칫Ϊ봫ͳƷȣתﲢû
գעղƷʽڴˣ＼ȡʵ
ʵͬԭѡĻ·Ǿɵļ֮£
תＰƷʵʩ޲ʵʵͬԭ޲ȷ
＼ĲƷ·ʵϣ2080
ˡ֧＼Ļ̬ȣתƷʩ
κβļܸӶڱ֤ﰲȫǰ£ٽ＼
ҵķչ

;ʮĩʼת󶹡޻׵Ʒ
׼ҵֲǶתĹע֮࣬Ҳ˱
Իڿѧ뷨ɵ·ԭ򱣳ģת
ƷзͲҵδӰ졣

رֵָԭеĿѧԭҲԭе
ģȷعǡɿĿѧ
Ŀѧѧ֪ʶж⣬κѧͬ
ϡƷ·γɹУѧͬ巢ҪúӰ
죬ر＼ԭ·ʱ

ܽԣתΪһũҵ＼﹤̼
ûر֮תǶԴͳķչͲ䡣ν
ȫ򲻰ȫȡʹáҲˣòƷԭм
ܡӦڶֲƷָ⣬㷺Ӧҽƺ͸ض
ܵ΢档ֻޡ͵ͲƷǵճ
ضȸǶתʳƷĹעʵϣת
ӦԶԶڴˡתѾΪִ＼Ҫɲֶ
ӦС

תƷ֪밲ȫ֪

ΧתУǳĵһתƷİȫԡ
ѧھתＰƷûΣһи߶ȹʶ
ġǵκκ϶ǲɱģԿѧ
ͬǸ߶Ͽɡתͻ޺
Ľۡ

ʱڿѧǵճ֮Ĳϡѧڽ
תʳƷİȫԱʱ硰תʳƷİ
ȫԲȴͳʳƷİȫԵ͡ı֮ѧҺþ
԰ȫһּƷǡǡǺܶԼϤĲƷʱ
׷ġӢʼѧ1985귢ġѧ
һѪָڶĹ񣬻Ǹеճ
պͲȷԵıԣѧҪɲִ֡᱾
ҲǷᡣܶ༼ͲƷաҪ󡰾԰ȫǲʵ
Ҳǲġ

תǳơתʳƷҪءߡתʳƷ
Ƿȫ޶ۡǰһȷķϻ͹߼
֤תʳƷ޺أҪе㲻ʵʣΪҪ
ȫųһֿԼ޷ﵽġ

ʵϣȻʳƷɫʳƷͬųкĿԡһֺĿ
߼Ӧǣûȷ֤ݺŷɱгϵת
ʳƷҪӦȻʳƷкҲǰڽת＼
ʱѭĿѧԭĻ߼ֻдڿɾѧ֤ʵķʱ
ſɶתƷʩӶĹܿء߼ԣȻһ֡
ޡԭҲǷǴճжϷʽһ˼·
תʳƷѾԴͷ϶ʳƷͻİȫ˳ֵı
ϣ˲ӦԡС֤סҪԴ֮

תʳƷǷȫжۡ˵Ȼûȷ˵תʳ
ƷȫʵǡǡһûݵδաĪС˵ǣ
֪ԼũҩʹáӲ濹Ӫֵת
ƺ洦ʵϣΪֹδκοɿо֤ת
ʳƷʳö˻кڸռճеĹת
ƷͻΣ۵ڿѧǻվסŵġ

ܽԣתƷİȫԶԣճﾳ£ǿ˵
ǰȫģǻڶԿѧͼܻΡһƶΡƶ
εҪɻʳøֳг۵
ʳƷڳճĲƷͷֻΣ
ִὫ粽СԷպͲȷԵǡ̬ȣ
ճԽȥǰᡣ

 

ǵͳƺо΢ƽ̨ϣйתУԼ60%
ۣ30%ۣռ20%΢Źںƽ̨ϣйת
ռ77%ϢռȲ10%ڸֱϣԣ
ıҲռ

һ˵ҹֽ׶Σת＼Լ
֪ͿԵġķѣͬ۳й۵̬ȵĶ
أͬƽֳ̨ܴ

һ棬ҲʾǣתͲƷĹ֪ϣ
һѧ⣬漰һϵغӰĺĸ
ѾΪ¹ĳͨ
תȱĿѧ˽⣬ִܸ
Ϣ͹۵󵼣ԴĹǳΪ̬γɵĹؼء

£ڶԿѧͬ͹ܲŵƶΣ
һԻҵĹ֪۵Ĺؼ͸ѧͬ
ܻҲҪͨŬԼĿеĹţĿ
ǰйת֪ϵվʱġ

׷ڡƼо20172ڣ42-45ҳ

(XYS20170802)

˿(www.xys.org)(xys10.dxiong.com)(xys.ebookdiy.com)(fangzhouzi.me)

곷 ӱƴѧ鼰س

83յ 83գӱƼѧŶӷ
ƣں»༭NgAgo-gDNAѴӹڿ
Ȼ＼壬ѧУԺоɹѧ鼰
سŶҲͬⰴѧУѡһҵʵңͬר֧¿չ
ʵ顣

ʱ83գȻ-＼ƣغŶ2016
52շڸڿġߺҲͬʱ
дڿнһֱ޷ṩʵ鷽ظͼ4
ʾĹؼǾо

ڡŶӷУŶӱʾΪȷѧоϽԣ
꼰Ĺͬ߾201652ڹڿȻ＼
(Nature Biotechnology)־Ϸĳأͬʱһоظ
ԭ

ʾŶͬⰴѧУѡһҵʵңͬר
֧¿չʵ飬֤NgAgo-gDNA༭Чԣʵ
ӦС

⣬ڸѳ壬ѧУԺоɹѧ
س

(XYS20170802)

˿(www.xys.org)(xys10.dxiong.com)(xys.ebookdiy.com)(fangzhouzi.me)

Ȼ-＼ۣǸ˵ʱ

һͨArgonauteøʵֻ༭оأʾķ
ͬȫýʱҪԡ

ڣ꼰ͬ³˷ȥ5µһƪġĳƣ5
ữDNAμ˾øNgAgo˫ѣʵֶ
ı༭һԱļȤýľ౨
Ǻܿ죬ءͺ罻ýȼ֮£йظоظԵ
ɿʼѸࡣȥ11£ˡ༭עEditorial 
Expression of ConcernѿнЩظԷĵǡΪ
ս飬оС˸ʵݡ񳾰䶨
ҲصʵΪNgAgoĹܶĴʱ䡢
֤

ƪȥ귢Ӱôŵ˵ҲΪ
ĵԴййý׷׽бԴһȫ»
༭ϵͳķ֡һƪйȥ걻ģý⹫˾
ģMeltwaterʾķ£н4000ƪ
صű

NgAgoĺ䶯֮пܲ䣬ȡCRISPR/Cas9༭ϵͳ
֮һϡNgAgoһĿнл༭Cas9ҪĿУ
ҪһʶPAMУңʼݻʾ
ƣȶԸǿDNACas9õRNAǿԣ
ٻ༭ѰУڻ鸻GCĻԣԼʹõԼ
ںϳɺʹ

˵һжȥ̫öţôȥ
ԽԽʵ޷ظĻ༭ܣ㿪ʼ
ˡڸֻ༭ϣ͵ʼУƪĳΪ
֮һܿýע⣬йظóʼЧԵ
ʼ档ڲͼɸûзֺĵ쳣
ݵλⲿҲͬ۵㡣

ڴڼ䣬Ȼ-＼һֱн籣磬עΪظ
ĳŬգڱ༭ǵЭ£Сĳɹγһƪ
ķģͨͬ飨Nat Biotechnol.34 768C773 
2016ЩݣǾгֵȥѶĿܴ
⣬ǽʽġ༭עڸƪڵַϣ˾ٵõ
ڵλߵ֧֡

ҲѯǷԽнΪظǵĽǣ
ȥ12£꼰ͬ£⼸뱾ϵĶоС飬ṩ
ݣѾظNgAgo༭ԡʱ༭һλⲿ
жЩ̫㷢׼ˣǾЩԭʼ
ߺµоСʱռ֧۵ʵ֤ݡ

ڣԭķѹȥһ࣬˽⵽˵ɹ
ʵĶоС飬޷ǿʼݣʹﵽɷˮƽ
Ƶģר˵ķж꼰ͬṩ
Էʼ㣵֤ݡȷźĳά
ѷм¼Ե

ƪйNgAgoķǿй̵Ľǿʼ
ıһǹĿйͬط˼飬ʶ
ǱڵĴԴ֤ԼŻ顣ڱУжλҵо߸
ѷʵ鷽ĸϸڽм飬ɼ¼ʵжķ
 Protein Cell 7 913 2016 Cell Res 26 1349C1352 2016 
PLoS One 12 e0177444 2017

ƪNgAgoҲʾ罻ýסȻЩƽ̨Ѹ
ѧĿܴڵⷢҪáǣҲ̧
ǵԤڣΪйƪĵֱ˵ԿٽġȻ
NgAgoĸ޷ڼڻ򼸸ھܳģԭġ
ʹǼ򵥵ʵҲҪ׼ʵʩͽֵ⡣
ǣЩпظоˣ丶Ŭòر
Ĺζûʽֺ֧֣á

ѹϣõ١ȷ𰸵ȫý͹Уķͬ
ƺ˾ɥǣ漰ѧʱûȷĴ𰸡
оظʱһ֪ģǾҪʱƪй
NgAgoĶԣʱˣѾ˵ˡ

壺NgAgoDNAĻ༭

ߣ߷壬Х壬ǿͺ

Nat Biotechnol.34 768-773 2016 201652߷
Ľӡˢ20161128߷ࣻ201782ճ壻doi
10.1038/nbt.3547

ڿнһֱ޷ṩʵ鷽ظͼ4ʾĹ
ǾоڸͼУǱ˵5ữ
DNAΪNgAgoNatronobacterium gregoryi ArgonauteܹЧ
˫ѣϸб༭ȻʵҶŬ
Protein Cell 7 913-915 2016 Nat Biotechnol.35 17-18 2017 
Cell Res.26 1349-1352 2016 PLOS One 12 e0177444 2017 
ûжظЩı档ˣڳǵ棬ά
ѧ¼ԡǻоȱظԵԭ
һŻʵ鷽

(XYS20170802)

˿(www.xys.org)(xys10.dxiong.com)(xys.ebookdiy.com)(fangzhouzi.me)

ѡɣӷĵڿ15

Դ

ʱ83賿ӱƼѧں룬»
༭NgAgo-gDNAɡȻ-＼ء

Ŷڳдڿнһֱ޷ṩ
ʵ鷽ظͼ4ʾĹؼǾоȻʵ
ҶŬûжظЩı档ˣڳ
ǵ棬άѧ¼ԡǻоȱ
ظԵԭṩһŻʵ鷽

ͬʱȻ-＼ΪǸ˵ʱˡۣ
ڣԭķѹȥһ࣬˽⵽˵ɹظʵ
ĶоС飬޷ǿʼݣʹﵽɷˮƽƵģ
ר˵ķж꼰ͬṩݲ
ʼ㣵֤ݡȷźĳάѷ
м¼Ե

ͬһգӱƼѧŶӷᵽ
ڸѳ壬ѧУԺоɹѧ鼰س
򡣡

ἰŶͬⰴѧУѡһҵʵңͬ
ר֧¿չʵ飬֤NgAgo-gDNA༭Чԣʵ
ԻӦС

1974꣬ΪӱƼѧڣƱҵںӱʦѧ
˶ʿͶйũҵѧԺйЭҽƴѧȡòʿѧλ

ƪѧ硢ýΪŵģ15º
г¡ڼ䣬ʼղԸʵ¼ʾʵ
ظڲϸĽʵЧʡô15¼䣬ѧ絽׾
أţwww.thepaper.cnˡNgAgo¼ʱᡣ

201652գŶڡȻ-＼߷ΪNgAgo
DNAĻ༭ġУŶʹNgAgo-gDNA
ڲ鶯ϸϵ47λ100%Ļ༭ЧΪ
21.3%~41.3%պŶӵʵüЧ֮ߣС
ħ֮ƵCRISPR-Cas9Իضλ׼ȷ޳ȡ

2016526գδռ䡱̳ϳˡӿѧǶȷ
¡ӣΪĴAgoϿвͨ˺ڰٶɡ
֪̳ϣɲϳ֡

2016527գӦѧﶯ̬ѧκʤ֮
Ժѧ档ʱPPTʾѾָϽжദ©

201667գ༭оӦáΪɽѧٿ
һΪαμ˻飬档굱
ͬɡ

2016628գΪ·ĺϻظѣϵͳ
ᡰʹҲͬĿǰNgAgoϵͳȶ2.0汾ר
Żѷš

2016630գӷɺ꣺˵ڱ
Ŵı϶ǿĿǰNgAgoǳ桢Ҫ߳ʵ鼼ɡ
Ƴ2.0Smart档Щ˵ìܵġΪ
ֻǸӵתȾʵ飬T7E1ͲҲֳɵļҪ߳
ʵ鼼ɣṩĲӦǲѱظġ

201672գзԭĵУ˽Ϊϸ
ӦǿNgAgoϵͳȾرУʵʱҪм֧ԭȾ
ʵ黷ԵĽ顣

201677գӱƼѧƼΪ2016ȡѧ߽ƻ
ѡѡˡ

2016713գ굱ѡΪӱʡЭϯ

2016721գйѧԺϺ񾭿ѧооԱԼ΢
֤˺š󵼡Ϸģʾʵظʵ飬Ż
Ŀǰʵ뺫еĽԶṩ
ظNBTµNgAgoŻNgago2.0smart汾ȵȡ

2016721գĴǹѧĻѧҸ̹Gaetan 
BurgioϷġҵNgAgoԼ715֮ǰ
ʵʱóĽۣʾϸϵ֤ʾNgAgo-gDNA
л༭ļ󣬲Ҫ󺫴깫ԭʼݺʵ

Դˣڽţwww.thepaper.cnɷʱʾƺ
һӦҿ£ţԵ˲С

2016730գߵȿѧίԱᣨCSICĹ＼
Centro Nacional de BiotecnologiaĿѧ·˹Lluis 
MontoliuĳƣѾֹͣйNgAgoĿͬʱ
˲Ҫ˷Դ

201682գȻ-＼־ӦýʾȻ-＼
κιĵǶԴؿǡ
оϵʾ޷ظоռȶ¡

201683գ»籨ӱƼѧʾһʱ
꽫ȡʵʽ֤ʱȨ֤

201688գȻ־˹NgAgoűظŪһ
ʿNgAgo༭Ӿ硷вԸ͸¶ĿѧȻ
־ͨѶԱŵ˹David Cyranoski֤ʵʵĿظԡ

ýɷʱʾȻ־ıһΡ
̸߿ŵ˹ǡԱʾȻĽǣ
ʵǿظģеܹе̸
Ѿ൱ǹӦˡ

żдʼѯʱŵ˹ظƣƪǱ
ѧģӦñΪʵظ֤ݡдû
κζظʵ飬˶ε޷κεһϢ
ıȷʵӳǹںʵظвͬĹ۵㡣Ҿ֪
鵽ڻûн

201688գӪϢAddgeneύ°ϸ
ʵ鷽Ӧע⡣

º˹ѧĲ٤ţ¾ɰ汾
ͼظʵ飬ʧܸա

201689գӱʡί˺ӱƼѧ༭оĽ
蹤ĿĿܽ2.52ƽףͶ2.24Ԫʽ
ʡʽš

2016819գʮλʦӱ ʦƺš

2016831գйɹΪӱƼѧ༭
Ĳɹ豸Ŀб꡷Ĺ棬ĿԤ1958

201696գӱƼѧѧУ񷢱ݽ
ѧУӵһڽѧ渺ڿڴµĽʦ飬
һ񺫴һʦ

201699գȻѧίʵٱĻ༭
ʵɿ鱱ѧ㡢廪ѧ³סͱѧо
۷֪ѧҲ顣

2016921գӱƼѧ༭оĲɹ豸
Ŀʯׯ꣬ԤΪ1958

2016923գļҲͶĹ˾Ŀӱʡ
б޹˾ύɺΪĿڲɹûִԭȹʾ
۷

2016930գӱƼѧƼΪƼ˲š

2016930գȻѧίվʾġNgAgo-gDNA
༭Ӧ̽Ŀ100ѧ20171¿ʼΪ
ꡣ

2016108գܡƼձɷãơΪʲôҪ֤ף
Լв𣿡

20161010գѧѧѧԺκʤ13λйѧ
ýϹ޷ظʵһ±ʾϣܹ
ԭʼݣںӱƼѧصλ磺Ȼѧ
ίԱᣩѧ顣

20161111գйѧԺܵӢڿϸоCell 
Research߷һƪͨѧصʵҸ͸
ѧѧѧԺоԱȵġ±༭šʹNgAgo֮Ȼ
NgAgoԣûзκλ༭

20161116գ20λѧڹڿϸ
ѧͨѶNgAgoʡɺŶӸĵĿظԡ20λ
ѧڸԵʵҽظʵ飬ڲͬϸϵ޷⵽
NgAgoĻ༭

ŻӦƣظһ⡣ҷһ
ƪ²ûнЩ⡣ѧкܶǲ֪ġеʵҲ
ظظԲԺܾظһݽḶ́ǸżȻ
ȻĹ̡

껹˵ԴӱظԵ⣬ҾҲߵϸ꿪ʼ
ˣһʼȷʵظ⡣ߵϸѾ
ظˡм䵽ʲôͬΪһйߣҪʵķʽ
ӦһͨѧڿӦġ

20161123գȻ־ٴαNgAgo¼ƪΪ
NgAgo༭ͬıУƣѾ
һ⣬ܱ˺ӵΪʲôظʵ
顣ڲ˵Ϊйý˵κλ

20161128գ¹ͺѧڡȻ-＼
ͨ£ͬ˸ʵ޷ظͨͬʱģС
Ȼ-＼һƪ༭עѶ߶ԭĽ
ģĿظԴеǡʾڵģԭߡ
Ϣ֤ԭṩǷǳҪġȻ-＼ʾ
20171µ֮ǰ飬ڹ½չ

2016122գѡѧ߽ƻѡ

20161215գȻѧίԱűʾĿǰ
ûؽչȻ-＼־20171µҪǵĵ
ȵȵǵĵ

2017111գ֪ʶȨַΪ֪ͨ顱ʾԺӱ
ѧںꡢ㽭ѧҽѧԺоԱХΪ˵ר
ArgonauteøΪĵĻ༭δڹ涨ڴ𸴹֪
ʶȨĵһ֪ͨ飬ר뱻Ϊء

2017119գȻ-＼ʾNgAgoϵͳظ
ݣھǷȡһж֮ǰҪоЩݡ

ţύȻ-＼ġ

2017119գӱƼѧڹ༭о
ŵάŹ˾Novozymes A/SNgAgoӦ뿪ɺǩ
Э顣ϢдNgAgo-gDNA༭ŵάŹ˾ϵͳ
ѾչֳǱŵάŹ˾ԽһNgAgo༭Ӧ
²ƷĿŨȤ

2017120գŵάŹ˾ӱƼѧ༭оĵĺ
УŵάűʾѾ˸
õһЩ󣬵ĿǰԴڷǳڵĽ׶ΣҪĹȷ
Ƿǵҵ񡣡δἰǷظеʵ顣

2017120գѧ߽ѧԤӡվBioRxiv³ƣ
NgAgoиRNAǺᣩ纫ڲϸи
DNAǺᣩݡȻ־͸¶Ѿһ
ʹNgAgoлĬר

2017520գƵŵ顷ĿΪں
ꡱȵĿعˡNgAgo¼ȥ

(XYS20170802)

˿(www.xys.org)(xys10.dxiong.com)(xys.ebookdiy.com)(fangzhouzi.me)

غϼ1792017.7.11-20

Verified account
@fangshimin
Tweets
15.3K
Following
38
Followers
124K

?Verified account @fangshimin  Jul 11

 ˵׹ΪŸѡл񹲺͹ϯ˵ɡлͳ˽
Ǹˣ֪ûΪѡձࡱ˵ɡձͳ¼˵ӡ
ͳǸڵİ׹Щҵʿ档
https://pbs.twimg.com/media/DEb50sFUIAAmTLA.jpg

?Verified account @fangshimin  Jul 11

 ս췢أԺֻȷͳ197ѡ48
ӮԾӡӣǲԺɣû
ӡͳѡҪҪͳȫɹ͵
ȷҲҪκԱͬ
https://pbs.twimg.com/media/DEfsI17UIAEe3ue.jpg

Eddie Cheng? @eddie__cheng
Replying to @fangshimin
ֻҪûдﵽ 60 Ʊġǿӵġ
Pew Research ݣhttps://t.co/rZHt6vvoLZ

?Verified account @fangshimin  Jul 11

 Retweeted Eddie Cheng
ûʲôϵ󲿷λǴԼûһȴû
ԺύĲֹ͵ֻҪ򵥶ͿԸͨڲ
Ҫˡ

Zhaoben Xu? @c6h6benzene
Replying to @fangshimin
Ժ߳۰ɡ

?Verified account @fangshimin  Jul 11

 Retweeted Zhaoben Xu
ǰԣΪҪ60Ʊֹۣڲ⣬50Ʊ򵥶Ϳ
ֹۣһ취ûС

пԺAVԺʿ? @atpwolf1027
Replying to @fangshimin
ϳûˣ͵簴˺˰ť±ͣˣֻҪ򵥶
ͨ

?Verified account @fangshimin  Jul 11

 Retweeted пԺAVԺʿ
ʵƻ˹ġ°ִڹ͵ٵ
ı¹ֻҪ򵥶ֹۿʼڹ
Ĵ߷ٵҲôӴ˲ԺͿԺΪ
Ʊ׶˾ԡ

gaovaqingshuang? @gvqs
Replying to @eddie__cheng @fangshimin
ȫ岽ôһ ԰ѵһ֮ ͵Ը
һlow Ϳˡ

?Verified account @fangshimin  Jul 12

 Retweeted gaovaqingshuang
еġȫӵĽҸ㰲ģֻ50Ʊ
ѸٱͨڵĹҪκƱ֧֣
͵ԱѱʱѸͨģͿˡѹִʲô
ٻ˵Ϊд۱ɵ˵ʲôʲô

gaovaqingshuang? @gvqs
Replying to @fangshimin
֧ԭĺܴۡܵˡ

?Verified account @fangshimin  Jul 12

 Retweeted gaovaqingshuang
޴֧ǷûκιϵϰԼת򹥻
ǡǡ֤Լ޴

Zhaoben Xu? @c6h6benzene
Zhaoben Xu Retweeted 
ȥһάٿƵļ飬ȷʵѡϼ򵥶ֹ
޸ֹ߷Ժѡ۵ż⻹Ҫ֮
ͣҲ֧

?Verified account @fangshimin  Jul 12

 Retweeted Zhaoben Xu
ҲһҪԺ͵Ÿҽƻڶ
ڼǰԴͬһӰ취ûСֻǷ۴
͵ԱȷʾҪͶƱûֻͨƳˡ
ҪһˣͿΪΪ

Ͼ? @zb_jing
Replying to @fangshimin
ûʿ޵ʿ

?Verified account @fangshimin  Jul 12

 Retweeted Ͼ
٣սֻйʮĿǰֻԱ޵ɣǲԱҲ
ˡһɣ˹

?Verified account @fangshimin  Jul 11

 նʼţмѼϣڲϵʼع⣩ô
¸˹̨ǲܲģȻʱܰʼйڶṩ
ϵĲɾִйýѧһС
https://pbs.twimg.com/media/DEf3q1VUAAAP31S.jpg
https://pbs.twimg.com/media/DEf3q1WVYAAqLyZ.jpg

clyet? @clyet
Replying to @fangshimin
Ե

?Verified account @fangshimin  Jul 11

 Retweeted clyet
ֻҪ͵ѳŹᣬûɶϣڵĹ͵ѱˮû
͵¸С

?Verified account @fangshimin  Jul 11

 ǺġûҲÿ
ģɵȥһᵯٵָμӸٱ˷˵
Ϊ˱ֺ﴿ԲѧӢǲӢĺ˼룬
С˵鹹½ֱҵˣݵòС
https://pbs.twimg.com/media/DEf_OyoUAAAdoGR.jpg
https://pbs.twimg.com/media/DEf_OyoUIAArdy1.jpg
https://pbs.twimg.com/media/DEf_OyoUMAA-8Gk.jpg

?Verified account @fangshimin  Jul 11

 £ֻץһҽǲ
http://mp.weixin.qq.com/s/0DxO2nvNdkTzytb8lCIxXA 

?Verified account @fangshimin  Jul 11

 ³ѸһϣڵģǶȺڣǵĹ֮࣬
跨עǵĸʱ򣬻׵ԣ
һƫԣӴ˼ѵꡣȻϺ
սɱĴȣΪȴǸҪΰĹ

Ҳ¸? @JunWu4  Jul 11

 ֱ³ѸйΰߡǳʱڣǳҪ³Ѹǻָ
йǡ

S.D? @justthinksd
Replying to @JunWu4 @fangshimin
ֱ³ѸǸϷǳɵɧҡǸʱնΪ׵
йڷ־ӣѹ߸Ȩսƣʱѵʲô
?ѵνԣξǹܰѹոܰѹұ?
ҪڻңڻǲҪԵģԾ͵ڻҡ

?Verified account @fangshimin  Jul 11

 Retweeted
ǰ³Ѹаô족СۺǸţ
ǰǱҲΣ³ѸҲˣҺο 

S.D? @justthinksd
Replying to @fangshimin
࣬ʱ򲻰?ҿɼǵߡ
˾þӹ˵?ˡ

?Verified account @fangshimin  Jul 11

 Retweeted
Сۺ֪ɶʱ򵱹ߡ 

?Verified account @fangshimin  Jul 11

 նӽ˺ֵʦԼı绤ʦҲʦԸ
֮ǰձҲҲԸͨŵ飬ֻüҵ
ʦ
https://pbs.twimg.com/media/DEgefCOWAAAaIWa.jpg
https://pbs.twimg.com/media/DEgefCKWsAAiBg-.jpg
https://pbs.twimg.com/media/DEgefCNXoAEatYi.jpg

look1m? @look1m
һӸרע76˺ţδŵˣ޳ܡ
https://pbs.twimg.com/media/DEgmfxrXkAEqgXv.jpg
https://pbs.twimg.com/media/DEgmgkfXYAEC41d.jpg

?Verified account @fangshimin  Jul 11

 Retweeted look1m
òƶǾ˺ţǿйע

?Verified account @fangshimin  Jul 11

 ھΪھҪƭԲͣط̰ҡƭ300򡱡
Ϊ񾲵Ƶھ裬ȴ±ƭú

https://pbs.twimg.com/media/DEhAtukXsAAn6H-.jpg

?Verified account @fangshimin  Jul 12

 ִҵҽ͡רҽˡǲӦٸһרҽ
https://pbs.twimg.com/media/DEhFtv6XgAEvbsk.jpg
https://pbs.twimg.com/media/DEhFtveXsAIYvWa.jpg

? @haiyazhizao  Jul 12

 ʦþûɹˣѾ36ˡ

WINSTON? @WinstonRecall
Replying to @haiyazhizao @fangshimin
涺ﳣ20ȣɹ˼

?Verified account @fangshimin  Jul 12

 Retweeted WINSTON
25϶ȡ

?Verified account @fangshimin  Jul 12

 ѿֻˡСɵ.һĸŦԼ
ĺֵʦˡʦԸˣ˵
ŦԼ塣Ҫ.յǸһֱΪǴŮ
ɵġǴˡ
https://pbs.twimg.com/media/DElCzb3UQAApXd2.jpg
https://pbs.twimg.com/media/DElCzb2UAAAiYNq.jpg
https://pbs.twimg.com/media/DElCzb0UwAAkP_l.jpg

?Verified account @fangshimin  Jul 12

 ŻԭŵͼСʼŵϵ2013
һزƻڶزĿΪ°Զ㾭Ʋõ¸
ĿԴպͶϣȡƲáСǣߵмǸ
ӵľˣǸʦһֱ˵ȡƲá
https://pbs.twimg.com/media/DElP0tTUAAAq02f.jpg
https://pbs.twimg.com/media/DElP0tRVYAAAkGJ.jpg
https://pbs.twimg.com/media/DElP0tRUwAAMSuG.jpg

?Verified account @fangshimin  Jul 12

 ڳһƪûӡ˹ʱдģƬҲ
յġѧhttp://mp.weixin.qq.com/s/qFTQR8N5iGdC1BlhGWg1LA 

?Verified account @fangshimin  Jul 12

 С˵ձ˶ʦһ²֪顣м
֪ġȥ6£˵¶ϣУʱС
ʦļ󡣺˵֮С˵
ʦûṩ鱨˵ȡԭ
https://pbs.twimg.com/media/DElogNiUAAAFsVN.jpg

?Verified account @fangshimin  Jul 12

 Ѿûֹҽˣҽ۵ĺշϼ
https://pbs.twimg.com/media/DEl0RVhVwAAy2A1.jpg
https://pbs.twimg.com/media/DEl0RVhUQAAbymt.jpg
https://pbs.twimg.com/media/DEl0RVkUMAAYccF.jpg
https://pbs.twimg.com/media/DEl0RVkUAAEP4HA.jpg

?Verified account @fangshimin  Jul 13

 Աµ꿪Ż | ӿѧġ۰ʣɻ
ļhttp://owls.taobao.com 

lawlee? @mylawlee
Replying to @fangshimin
ǩ

?Verified account @fangshimin  Jul 13

 Retweeted lawlee
˿ݿǩ棬ǲۡ

?Verified account @fangshimin  Jul 13

 ̸ڰ׹ͳ칫Ҽշ
https://pbs.twimg.com/media/DEmS-QIVoAAt0xi.jpg

?Verified account @fangshimin  Jul 13

 ³ѸʱҲÿˡ£Ҳ⻰ӺΪû
ߵǱӵĻ죬Ϊݺ˶ƭ
Կˡ

?Verified account @fangshimin  Jul 13

 ѧоԱû༭ϸDNA롢һСεӰ
ʷϵһӰ
https://pbs.twimg.com/media/DEqOUFWVwAAZXPi.jpg

?Verified account @fangshimin  Jul 13

 ѡҽҩչսԹ滮Ҫɨһ飬˹涨ҪԱֻ
ҽֻҩܽչҽҩҽ۾
https://pbs.twimg.com/media/DEqVzLjUIAAR705.jpg

?Verified account @fangshimin  Jul 13

 FMĿӽѧ168:Чк
https://fm.qq.com/luobo/radio?_wv=4097&aid=rd001USq1949X1mp&showid=rd0
04990EX1F4W4J&sourceInfo=adpos%3D20034 

?Verified account @fangshimin  Jul 13

 £ѧ
http://mp.weixin.qq.com/s/badrVeJsPajBfhJs5rYO3A 

?Verified account @fangshimin  Jul 13

 룺һǸıǮǾ̸֢֮ڿҽ
ҩûãڼظĽⶾܵǼ
ɢˣ֪˵Ҳˢ

?Verified account @fangshimin  Jul 13
 Retweeted
ʯҥҸܣȻǲָģ


? @liujingduo
 Retweeted 
ű҄hݴǡʯ˽܊WvǷˮ֣Ȼ᷽
܊ܡ҆ӑMM߀ҧһ{
lйՓ

?Verified account @fangshimin  Jul 13

 Retweeted 
ҥôֱ׳ôɾģҶʯ͵ҥ̰Ĳ
һ¾УҲʲôйۣ֪ΪʲôƲ
ҥ˺ô˶ûѧأѧ෴Ƿ


? @liujingduo

@fangshimin 㮔܊ʯ͡댦fcһҪҮ
]Yָ؟e^ĸܣ㲻䡣ȱ½oСec
¡

?Verified account @fangshimin  Jul 13

 Retweeted 
޳ҥҸܣȻҪôˣвҼСˣ
ġ¡ġ¡Ҳ˵ɡȱ¡Ž֡

Ħ? @sscc09101
Replying to @fangshimin
ʯʼϷʾʱѲ

?Verified account @fangshimin  Jul 13

 Retweeted Ħ
ʱֻų̂ʯҵҪұܣȻûдӦҲ
ꡣʯºַҥйܣҪʮ
֮ǰǸ򹫲żܾǸȹһżȡ壬
Ҿ͹żȫġ̨ʯΪҸȺҥҸܡ

FtԺͯ? 
@ponney_zh
 Follow

Replying to @liujingduo @fangshimin
ʯԼ{ӛߣ
܊Wvм٣
ԓģ
Լȥ{飬
ڱ˴˲Ϥr£
˽]@Cɽͽoӡ
Ƿӣ
Ҳһӕ|Ŀĺڡ
wf
ʯ@úܲص

?Verified account @fangshimin  Jul 14

 Retweeted FtԺͯ
ʯ֤ѧⱾҲʲô˵£ҲûϡȻ
Լйҥйܣ̨ӢԾΪȺ
ҥҸܣΪҥĶ

?Verified account @fangshimin  Jul 14

 Retweeted
Ժǩǩûиʲôϵɢ˵˹ܺװһӢ
Դ;ǹˣ 

? @changzhanglao
Replying to @gaopx @fangshimin and 3 others
֤һ£оҦɢˣʵ˵
۵Ҧҥʵ
https://pbs.twimg.com/media/DEq-WOvV0AAASAh.jpg

?Verified account @fangshimin  Jul 14

 Retweeted 
ϸˣɢ˵оĻȷ˵ģ˾
ʵֻƱ˵ûڸܲǩԲ֤ܰݡ

? @changzhanglao
Replying to @fangshimin
ҿˣɢ˵һƷϵѶΪ
Ͷ塢ѷΪɾˣǸܲϡӦͨ¼ʽ
أȻǩ֡
https://pbs.twimg.com/media/DErt1V4VwAA1zc2.jpg

?Verified account @fangshimin  Jul 14

 Retweeted 
ǶԵģûע⵽иαġҥɢ˲˸ܣ
ҳڸܲǩ֣˵Լܡ⡱ôС˵Լ͸
һζ

?Verified account @fangshimin  Jul 14

 Retweeted
ΪʲôҪǩǩûǩɢ˸ûʲôϵأǩҲûɶ
˵ģǩߴ󶼹úúõģֻ֯ǩ߲űˣֻ
ɢ˸ܵĹ

? @changzhanglao
Replying to @NZqingdaofu @fangshimin and 3 others
³ѸСеһζԻۣ
ףңǵǿɿĺˣԼֹϵ鶼û㣬
ô˸ȥˣ
ңдôǸܣ˵Ϊѽ
ף㲻˵֪
ңʲôһû˵ѣҲֿסˣ

?Verified account @fangshimin  Jul 14

 Retweeted 
ɢ˺ǾΪ¡ˣѱˡ
ֻûжпС

?Verified account @fangshimin  Jul 13

 ˹ͳڡȫѧᡱݽѧ˵Ϊ쵼Ҫ
ͳǫƷʣûЦ
https://pbs.twimg.com/media/DErOg9BUAAAD45a.jpg
https://pbs.twimg.com/media/DErOg9CV0AAoYKu.jpg
https://pbs.twimg.com/media/DErOg9DVoAEG-zB.jpg
https://pbs.twimg.com/media/DErOg9CUAAAxG0c.jpg

?Verified account @fangshimin  Jul 13

 ȫѧҶȫůϼ޴Ǳһ磬ֻ
ѧҸġûбҪģҽҪ
https://pbs.twimg.com/media/DErVlwyUwAAcYru.jpg

Ian Johnson? @iandenisjohnson
"His was not an active decision to die, but a willingness to do so:" 
my piece in @nybooks on #LiuXiaobo's legacy
https://t.co/7xogdH7jJY

?Verified account @fangshimin  Jul 14

 Retweeted Ian Johnson
 means "My death serves a purpose" instead of "This is the 
place where I should die".

Ian Johnson? @iandenisjohnson
Replying to @fangshimin
Thank you--I took the translation of  from Jonathan Spence's 
"Gate of Heavenly Peace." But your translation is clearly better.

?Verified account @fangshimin  Jul 15

 Retweeted Ian Johnson
Literally it means"die in the right place", but it's a very old idiom 
(coined in the 6th century) with metaphorical meaning.

Andy Tu? @canhuatu

Replying to @fangshimin
е֣ûġʦǽָ㷽ӵķ롣ķ룬
ͨ˫ʤ

?Verified account @fangshimin  Jul 15

 Retweeted Andy Tu
ǽ÷ˣȻڷˣֻǽˣ
ȥˡ

?Verified account @fangshimin  Jul 14

 ǵԮԪͨѶ鳤ѩ÷𣿴ԪԼ
һɸġӷǷʱץˣ永ڡ
https://pbs.twimg.com/media/DEvR6QHUMAA1_D4.jpg
https://pbs.twimg.com/media/DEvR6QGUMAA6npN.jpg

?Verified account @fangshimin  Jul 14

 ջҪҽƭųҩ䡰ָܡϼ£ҽ
ôˡ
https://pbs.twimg.com/media/DEvb36fVoAUWCos.jpg

weiwushan? @weiwushan
Replying to @fangshimin
ںܶסֹѪȵȵгҩҩɷ֣ôڮ
ҽϣ´ǰҽҪܿҩǺǣ
ҩǵԺǧҩ

?Verified account @fangshimin  Jul 14

 Retweeted weiwushan
ÿԣԼŶսԺǧˡ

?Verified account @fangshimin  Jul 14

 Ϊʲôᡰ
http://mp.weixin.qq.com/s/7izCB7BPQoiLnDwvb-9BbQ 

?Verified account @fangshimin  Jul 14

 ЩԳҮв׷ߣкü׷档
https://pbs.twimg.com/media/DEv5DlOVoAAqJgv.jpg
https://pbs.twimg.com/media/DEv5DlaUQAE0wbd.jpg
https://pbs.twimg.com/media/DEv5DlBUwAAj3wv.jpg
https://pbs.twimg.com/media/DEv5DlWUAAASk3X.jpg

?Verified account @fangshimin  Jul 14

 ɢ˵Ժǩǩǰи˵ǩܶ춼
ǩˣûǩǲΪ֪֯ûܣ

?Verified account @fangshimin  Jul 14

 С˵˼һǡ͸ģȴֱڳ治ֻ
һʦϯһ˵͡ԭ鱨عҲڳһ˫
治ֹһΣиϱڳڰ赱ڳ޷ͳ
ıֵòѿֻˡ
https://pbs.twimg.com/media/DEwZdRRU0AABAqT.jpg
https://pbs.twimg.com/media/DEwZdROUQAE6FCT.jpg
https://pbs.twimg.com/media/DEwZdRNUMAA8Rvy.jpg

Ke Chen? @cktears
Replying to @fangshimin
ѶŮð֣˵ôûÿ顣ϣ
ϣ
https://pbs.twimg.com/media/DEwS-f4UAAASads.jpg

?Verified account @fangshimin  Jul 14

 Retweeted Ke Chen
ϧ鱻༭̣ˡѧèͷӥĿֻǾĴûָ
ԭò񲻹ˡ

?Verified account @fangshimin  Jul 15

 ԣʮһҵҽƱϵͳӢŵһ
https://pbs.twimg.com/media/DEwkqanUwAA_nGM.jpg

Zuyuan Zhu? @ZuyuanZhu
Replying to @fangshimin
Ӣŵһ  Ц

?Verified account @fangshimin  Jul 16

 Retweeted Zuyuan Zhu
йЦӢţ

Dayin Ma? @dyne98
Replying to @fangshimin
⵹٣ܶӢйˣйĿϰߣҽѧʶܶ
ҽɷ֣Ӣҽƶȷǳðֻȥ˲Ӣ
ҽƵԽͲѡ

?Verified account @fangshimin  Jul 17

 Retweeted Dayin Ma
й߸ȨҽƵģȻӢҽƶȲã
ţӢôصҽìܣôҽƭô˵ҽôû
ҽ

?Verified account @fangshimin  Jul 15

 ³Ѹйľ类ǹڴˣྭ飬ѾҪ֤
ĻһС⡰һСȻοȸŵúһ
㣬Ժ¯һʹҪǱӦԶҩҽ
һȫ׷ѣΪڴҴʱ򣬺Ծӷ


?Verified account @fangshimin  Jul 15

 ܹ
https://pbs.twimg.com/media/DE0bjViUMAA4_yI.jpg

?Verified account @fangshimin  Jul 15

 Ȼǰ׶عԱ֪ëάӭҲ̫ˣô
ĵͼӲˡǰ׶عԱάӭӢг
http://mp.weixin.qq.com/s/eRKDrVZPNDudeIWkWZNTtA 

?Verified account @fangshimin  Jul 15

 дƪµʱ򣬻û˵ǽûġ
Ǻ֮ġ·֡񻰺͹
http://mp.weixin.qq.com/s/oLwfbDJF3dtkrXnkBBgObw 

?Verified account @fangshimin  Jul 15

 Ĵ˵ĴֻϷɣѧɡӡڰ
֤Բʾȷص3˵ůйƭ֡
ʵҲࡣ
https://pbs.twimg.com/media/DE1BWkqV0AAVRwv.jpg

?Verified account @fangshimin  Jul 15

 ǵö·ݼиŮͨairbnbסȴԡ
ˡΪɾסڼݹƽҵסֵĵ£÷ͬ
⳥5000ԪȥĻγ̡
https://pbs.twimg.com/media/DE1PlHZVwAE_J3w.jpg

?Verified account @fangshimin  Jul 15

 ͳ̸йͳʣй8000Ļйٷֻ˵5000Ļ
3000꣩йͳʴ̣ܶйԳйս
гܶܶ͵شͻʵԺйΪҲ
ụ̂гϵһֱѺõģԽϵͬ
https://pbs.twimg.com/media/DE1WjipUIAEnyNQ.jpg

?Verified account @fangshimin  Jul 15

 ƶȽΨһŮԻߡ˹̹ѧ׶ٰȥ
40ꡣʵ񣬰սġ޸ݿѧң
ͷϢʱصʱͷƬ
https://pbs.twimg.com/media/DE1d4MhUIAIC_h_.jpg
https://pbs.twimg.com/media/DE1d4MhVoAABQeY.jpg

Yongjun Liang? @yjliang_my
Replying to @fangshimin @RealJingyan
ʱֽûͷ
https://pbs.twimg.com/media/DE9dSxsXcAA8e-l.jpg

?Verified account @fangshimin  Jul 17

 Retweeted Yongjun Liang
˵ϢͼΪ֤ʱֽ˵£

?Verified account @fangshimin  Jul 15

 ҽշϼҽŹҽҩѧи
ҵ벻ܵҽ
https://pbs.twimg.com/media/DE1oUTfV0AEKoaP.jpg
https://pbs.twimg.com/media/DE1oUTfVwAEge-1.jpg
https://pbs.twimg.com/media/DE1oUTfUAAABK_w.jpg

δδ Ai Weiwei?Verified account @aiww
δδ Ai Weiwei Retweeted
ܵĻɲš˵ʽ뵳Ĩڲʽ޵
۲ӡ˵Ҫ˵Ҳϼ˵ֲ֮
ɡ 

?Verified account @fangshimin  Jul 16

 Retweeted δδ Ai Weiwei
óѡ񡱣ûҲҪһͲáһҶʿû
ԡ

?Verified account @fangshimin  Jul 16

 רҵרҵ¡йǲһõģ־ʦ
ɲרҵ£ʳеģֻҪҽָ
ݾжϡ
https://pbs.twimg.com/media/DE5g1ZNV0AEOxLB.jpg

?Verified account @fangshimin  Jul 16

 Ҽֵ¼130
https://pbs.twimg.com/media/DE5ogWkVwAEaf-T.jpg

?Verified account @fangshimin  Jul 16

 ںǲҺģѾȥˣںꡱ
https://pbs.twimg.com/media/DE5wCYxU0AAQ1VH.jpg

Triumph of Trees? @treeumph
Replying to @fangshimin
The King's dead, long live the King!

?Verified account @fangshimin  Jul 16

 Retweeted Triumph of Trees
һҺںŵġһkingָϹڶkingָ¹
ɶɱȵģѵףСꣿ

? @W0B9D9tfvow4kXr
Replying to @fangshimin
ڳ

?Verified account @fangshimin  Jul 16

 Retweeted 
಻ڳ꣬long liveڳ꣬ǻᷭǽ˻ǡ

Ѧ? @TcvC2c4pqiLJ9VZ
Replying to @fangshimin
ӣ˼˵࣬ôɳˣ

?Verified account @fangshimin  Jul 17

 Retweeted
һ˻ķǽǡ

LAURENCE? @LAUREATEM
Replying to @fangshimin
Ӣۼϵ""Ҳ

?Verified account @fangshimin  Jul 17

 Retweeted LAURENCE
ֵѡࡱlong liveֵࡣ

?Verified account @fangshimin  Jul 16

 ÿ죬бΪӱոֱһ
ԸɱǱȵıɱΪʲôªϰ
http://mp.weixin.qq.com/s/V1mgFmdxY8eTc-6B9lAKWg 

?Verified account @fangshimin  Jul 16

 ֪ʶӡʼˣҺüƪĶյû
ȥ
https://pbs.twimg.com/media/DE6KTYlVoAAqWxh.jpg
https://pbs.twimg.com/media/DE6KTYjUAAA76iQ.jpg

δδ Ai Weiwei?Verified account @aiww
һֱͨѶϵֱӣ
˵ֻǾܾһֿ֡ѡӽǲԸǵĲ룬
鿴ȥллϵۡЩͼ¼
ϼԸ´Ӽ򣬲ˣѸٺᡭƵ㵽Ӧ˭

?Verified account @fangshimin  Jul 16

 Retweeted δδ Ai Weiwei
֪Դ˳ѪͷǴͳܺ˭ͨѶͨѶ
ȻҲǾģûͨѶϵġֿѡǲΡ

Ʊ? @fybh6464

¿ĸ굶ֿʼ @fangshimin
https://pbs.twimg.com/media/DE6U0izXoAAbszU.jpg

?Verified account @fangshimin  Jul 16

 Retweeted Ʊ
߶߶ΪΪ¶Ӳò˼Цˡɢ
һֱǶɶʱˣ߶߶ԼϿģ

?Verified account @fangshimin  Jul 16

 1934˹ѧķԡ·Ϊɱ͵
1937һǧһ𱻼ǹСڴڼϵظŮ
дͼĲòѧ
https://pbs.twimg.com/media/DE6nve8U0AEq4uN.jpg
https://pbs.twimg.com/media/DE6nve9UIAA2Xjp.jpg
https://pbs.twimg.com/media/DE6nve9V0AAXlWE.jpg

?Verified account @fangshimin  Jul 17

 ͸ʱԺʿ³Ѹһýˣ޿ѧ
Ϊѧ̵ܽףܽ˼·죬ȻȻĳ˽
˵Ķͷǽˣһų ǵ
ҡȰѿѧŪǷǲѧҲ

Ian Johnson? @iandenisjohnson
Wimps
https://pbs.twimg.com/media/DE7aW46XkAAw0J-.jpg

?Verified account @fangshimin  Jul 17

 Retweeted Ian Johnson
That's why LinkedIn can do business in China while Facebook can't.

?Verified account @fangshimin  Jul 17

 ӿѧġ14ֿļ
http://mp.weixin.qq.com/s/VmHeyPQ_JGRiEv6gO4MeFw 

? @GnrZhao
 Retweeted 
ⲻǷȨ
ôĶ

?Verified account @fangshimin  Jul 17

 Retweeted 
ȨҵģֻȨˡѣ˻ҪĶġ
ʵҲǺ

?Verified account @fangshimin  Jul 17

 ܲؽڽ֤ϼƵ11ˣ˶߻ŻϥԹ
׵ıΪ10.2%ǲܲΪ3.5%ܲߣ˶ԱΪ13.3%
https://pbs.twimg.com/media/DE-tieXUwAAhEXQ.jpg
https://pbs.twimg.com/media/DE-tifLVwAEaNFd.jpg
https://pbs.twimg.com/media/DE-tieWUAAAcrr0.jpg
https://pbs.twimg.com/media/DE-tieZUIAAfPBf.jpg

?Verified account @fangshimin  Jul 17

 Ӣһ67긾Ůȥҽطһֻ۾27
۾Լ֪
https://pbs.twimg.com/media/DE-0Yl5UMAIPFhZ.jpg

? @zuoanqh
Replying to @fangshimin
ô֮ǰ鲻Լ

?Verified account @fangshimin  Jul 18

 Retweeted 
ǡӢҽѧ־ĲһϿдŽ¶ȥ

?Verified account @fangshimin  Jul 17

 ȥܾ꣬μ塣ˣֶٷ򸾡ټʲӡȡ
֮ǣӵʷĵһ塣».¬˹
ռڲĸ
https://t.co/lk2OXMNtWi

?Verified account @fangshimin  Jul 17

 ƪ²ǰѧ԰ˡӦðҵ𽥷
ڵġĽħǡ
http://mp.weixin.qq.com/s/tONpNZiqzwpzAt1Gn8Ju_g 

?Verified account @fangshimin  Jul 17

 ҳԽԽõˡ׹ŲСպͶ˼棬ȫΪ
ıֹ¶ߣûб¡շƣСȥ
˼ΪѼѡ鱨Ͷôɡ
https://pbs.twimg.com/media/DE_tZvoVwAAUFxg.jpg

?Verified account @fangshimin  Jul 17

 ڸ󸱲λô󲿷ֻţɫʵѵεģȦ
ѡԺδȷϵģɫǻδѡģǿп޵λá
https://pbs.twimg.com/media/DE_2G_xUIAABDW0.jpg

?Verified account @fangshimin  Jul 18

 °칫νְˣΪΪͳȨԼ
ǮûڹзܣڱЦ
https://pbs.twimg.com/media/DE_9lRCVoAEQU8K.jpg
https://pbs.twimg.com/media/DE_9lREUQAAFEDw.jpg

?Verified account @fangshimin  Jul 18

 ³Ѹţ˱Ӵмԣ˷ǰһ˵ϣ
̸֮ȫɨ˭˵ļȫ˺˭
˵ֶȫų⣬֮ܶǽĴͳСɵնȫŵ
ȥѧѧǹǵӣſµϣѿ

?Verified account @fangshimin  Jul 18

 ١FBIȷ˲μС-ܻȫ8˵ݣ8
ڶĺ顢زĴˡⷽǶ
ʦǰ鱨عֶ˵͡ز˺ͷ룬ⷽ
СայŮʹվѡŶܾǿ
https://pbs.twimg.com/media/DFD1ZR0VwAIjJQT.jpg
https://pbs.twimg.com/media/DFD1ZR0UAAACOn5.jpg

?Verified account @fangshimin  Jul 18

 غһͬʱڲͬԺ37ʯ͹˾Ҫ⳥
ΪʹûʯȼϵºƽɵĸʧͶˣ̲ݹ˾
Ҳǽ˶Ҫ֡
https://pbs.twimg.com/media/DFD9WIAVwAAW2Sf.jpg

Eddie Cheng? @eddie__cheng
Eddie Cheng Retweeted 
ҵ١ʯ͹˾ֻǿɣʹáĻʯȼϺ١ҪӦ
ʹȼϵķ糧Լɻˡ

?Verified account @fangshimin  Jul 18

 Retweeted Eddie Cheng
ʯú̿˾ֻǿɡ˵ģϵͳء෽λزȡֶ˲
ŹůĿѧ֤ݣӿůĴʩٽʯȼϵʹ
е档

Eddie Cheng? @eddie__cheng
Replying to @fangshimin
ЩΪ۶Դɵķ룬Ƿ⡣

?Verified account @fangshimin  Jul 18

 Retweeted Eddie Cheng
ҵͨɢĲشΣ۶
ɡ

Ke Chen? @cktears  Jul 18
 ߼ƣ̲ݹ˾ֻ̣Ѷ٣ֻ
۶ƷӦ......

Eddie Cheng? @eddie__cheng
Replying to @cktears
̲ݹ˾ΪкƷֱܺƭߡ
߻ǵĴˡʯ͹˾ĲƷ糧˾ȵȵġ
Ʒ⣬Щ˲ܺƭߡЩطסܺ
Ϊ糧˾Ϊʯ͹˾ΪߵĶˡ

?Verified account @fangshimin  Jul 18

 Retweeted Eddie Cheng
̲ݹ˾ϲߡǴǸݵļ
ߣȸ޶⳥

?Verified account @fangshimin  Jul 18

 µĵ飺ȫ62%Ẓ̌74%꣬72%56%ã54%
49%ŵϵĹ̩ǡ֡ӡȡɡͲ
¼ǡϵйձ䡢ӢʱĴ
ǡϽ̶ԽߡԽáԽԽŽ̡
https://pbs.twimg.com/media/DFEMcGDVYAAsX64.jpg
https://pbs.twimg.com/media/DFEMcGDVoAAIDtD.jpg
https://pbs.twimg.com/media/DFEMcGEVoAA5Ww3.jpg

? @UJTfCHGtiqjmZK2
Replying to @fangshimin
Ҹо鱨治ô׼ȷɣҾð˹ͽҲϰ
ߵĻйĵ̺ͷ̣ȰƲɱβ⣬

?Verified account @fangshimin  Jul 18

 Retweeted
һԴ;˼ҵĵ鱨治׼ʡ70%Ž
㻹òߣΪӢģֻߵڽ̣ 

Himura Kenshin? @wingian1986
Replying to @fangshimin
ըһƬڽĪøеľʿΪûڽ˾Ͳ
ҸЦˡ

?Verified account @fangshimin  Jul 19

 Retweeted Himura Kenshin
йò׸һվһ𣬰һЩǼˡһЩӢ
ͽ˵ҥ֪Ͻ̶ԽߡԽáԽԽŽ̡
ǵۣԭдš

eunz? @eunz8964
Replying to @fangshimin
ǵɹѹ˷ֹߣԼë
ѹ̺ͷֱ̣׹Ϊ򿪵ĵ֮š

?Verified account @fangshimin  Jul 19

 Retweeted eunz
еĻҲΪ׼ġ

?Verified account @fangshimin  Jul 18

 ҵġɹ׼http://mp.weixin.qq.com/s/vEiZVjwfvkZptzkhr4ly0Q 

?Verified account @fangshimin  Jul 18

 СʲʱԺǰ鳤͵ʱһк
ǰ˽˸޶ڷѣηȡÿȡһԪԱ
бɹ涨ȡһԪҪ걨Ӣһ·
ְѹ׷ڣԹȡ걨15¡
https://pbs.twimg.com/media/DFE3c4OUQAAmit7.jpg

?Verified account @fangshimin  Jul 19

 ŹԿ˭û˭䣬Ժôͻ
https://pbs.twimg.com/media/DFFIVbBU0AAYG-Z.jpg

? @jiayuqingfeng
Replying to @fangshimin
ͼΥ͸С

?Verified account @fangshimin  Jul 19

 Retweeted 
һԸҥ˵Ƕǰľϰԭϰˡ

־ΰ? @rbanLeLROcai58w
Replying to @fangshimin
ŹŹкιף
ǹʽʷ̿úá

?Verified account @fangshimin  Jul 19

 Retweeted
֪͸һʶûУԼʸӡʽʷ̿顱
ʶ

?Verified account @fangshimin  Jul 19

 ҹߣܹɫǣ롱кۣɫǴˣ
¶ֵɳҲֻǰΰǷˣѹ
ʵ͡
https://pbs.twimg.com/media/DFJDRdLU0AEmaGY.jpg

?Verified account @fangshimin  Jul 19

 ³Ѹ: һҹƫעڱ£Ƕ̫ͬУвϣ
֮ΪΪūˣûᣬȡԼĿǰ档

?Verified account @fangshimin  Jul 19

 £ͬǲԹϵ
http://mp.weixin.qq.com/s/a7zmmcv5j5rMbE3xFdZ_Uw 

?Verified account @fangshimin  Jul 19

 þûСʦϢˣæʵء
https://pbs.twimg.com/media/DFJoIOOUIAUg8Ky.jpg

?Verified account @fangshimin  Jul 19

 ֻ˹̣Լ̫̣ΪܳԵڶࡰʳ֮һ
йҲ˼ɻ䣬漰˹̵ıֶἰˡ
иӣͷ»籨£ǲҪ
һ˵Ķͷ
https://pbs.twimg.com/media/DFJ-nJgUwAAHtDF.jpg

?Verified account @fangshimin  Jul 19

 Ż˵ȡյ̱ˣֵй˲Ż
£Ŷԣ˵иСйյҲ̱ˣʲô˵
йñ֡
https://pbs.twimg.com/media/DFKHmWKVoAAJyuH.jpg
https://pbs.twimg.com/media/DFKHmWMUIAAHhBK.jpg

?Verified account @fangshimin  Jul 20

 ³Ѹñʺ࣬Ϊū֮ߴңȻǲģҪʮ
СģʹҵĽۣô׻ƵԼ˵ū
á

?Verified account @fangshimin  Jul 20

 ʨ鲼ޡŷжӡȣֻڷĳЩ
https://pbs.twimg.com/media/DFOOnMQVYAEFN8e.jpg

XuWanYang? @xuwanyang
Replying to @fangshimin
ӡȼɭﻹͷʨ

?Verified account @fangshimin  Jul 21

 Retweeted XuWanYang
ԵģӡﻹиС㡣

?Verified account @fangshimin  Jul 20

 вûôءӦ˻쳣дǮ
ϴǮˡ⣬ӢϴǮİԭ5·о֪
ˣ
https://pbs.twimg.com/media/DFOUcVZUwAAlqwt.jpg

Eddie Cheng? @eddie__cheng
ĸѧϵͳܹܽѧȡΣΪͨſ
ѧܱҵԭ򡣿ԺѧϴѧдһЩѧ
ϰΣxxx studiesͳˡ
https://pbs.twimg.com/media/DFOWb-rUwAAtY5F.jpg

?Verified account @fangshimin  Jul 20

 Retweeted Eddie Cheng
ֻǽѧĿѧˣҪѧġѧˣ
γҲҪѧġѧĿǺܱѧȥƾ
֮ʮѧйĿҪ˵רˣƶѧѧġ

?Verified account @fangshimin  Jul 20

 ǰ11ŵ48ꡣⶼΪ޵¡Ǹƭ֣
֮䣬𣿡޵¡Ƿƭ
http://mp.weixin.qq.com/s/IOVGf6Z_Qs-BcEoK-jjP0A 

ˮһ? @wayne_liu1
Replying to @fangshimin
ּ¼ƭ֣֤ƭֺףٵһλŵ
³ĸͼƬɣûȥΪ֪Ǹƭ֡

?Verified account @fangshimin  Jul 20

 Retweeted ˮһ
ʹٵһΣҲ˵ƭ֡

?Verified account @fangshimin  Jul 20

 ȥ3µ3£28ܽ1530Ԫ
йŵһ317Ԫй˻϶ȥģй
ʼúˡ
https://pbs.twimg.com/media/DFOzJ_BVoAIurrV.jpg

?Verified account @fangshimin  Jul 20

 G20ϣˣԼȥվͨ˷̸һ
СʱŦԼʱ̸ʲôأ˵̸¶⡣ǰСҲ
˵˼̸ֻ⡣ôҪͳһĽڣ
https://pbs.twimg.com/media/DFO_AKiVYAAFmb2.jpg

William Nee? @williamnee
Replying to @fangshimin
ֱӸĻйءͨĻԺ
˹ͷ˹ͯ ݸ÷ܾ
ڶ˹ַȨȴӵ˳ͷĶ˹ˡǣ浽Ļ˰
թĹԱ뾳ǩֹ֤ʹĽϵͳ

?Verified account @fangshimin  Jul 20

 Retweeted William Nee
ⱾǺܸЦ£ԶоƲãԼĹ¶Ϊ
з

?Verified account @fangshimin  Jul 20

 һŦԼʱǼţһϲɷãȻŦԼʱһһ
ָڷ̸˵ЩٻŰʩŰ
https://pbs.twimg.com/media/DFPIHdBVwAQ0vUz.jpg
https://pbs.twimg.com/media/DFPIHdHUIAAz2Zw.jpg

(XYS20170802)

˿(www.xys.org)(xys10.dxiong.com)(xys.ebookdiy.com)(fangzhouzi.me)

һΪ˰޷˿վĹ˽˿վĶ̬ؿ
˿ʼбxys@yahoogroups.com

ʼбÿ췢һ˿վ¸µϣÿµ׷һÿ
Ŀ¼ʼбĶμ롰˿֮ѡ
ʼбxys_friends@yahoogroups.com

ù䶩ġù䣬ܿ޷յ
Ƽ䣺yahoo.com, hotmail.com, gmail.com

ġ˿ʼбĵ䷢һհż

xys-subscribe@yahoogroups.com 

塢ͣ˿ʼбͣ䷢һհż

xys-unsubscribe@yahoogroups.com 

Ͷxinyusi@yahoo.comôıļͶ塣

˿(www.xys.org)(xys10.dxiong.com)(xys.ebookdiy.com)(fangzhouzi.me)

