                           ˿µϣ2016729գ

16.07.25-28ûи
16.07.29, ӡ̴ƽ𣿡
16.07.29, ӡͬðǼ٣
16.07.29, FMĿӽѧ74ҪHPV
16.07.29, תЭԭϯMontoliuʿ鲻Ҫͼظʵ
16.07.29, Աཨѧʵֻ1939ChernickĶѡ
16.07.29, ڵӾβ
16.07.29, lw56102̲׻θ
16.07.29, ͢ת</a
16.07.29, غϼ1252016.07.21-25

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FMĿӽѧ74ҪHPV

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תЭԭϯMontoliuʿ鲻Ҫͼظʵ

ĴǹѧGaetan Burgion׫дĽͼظӱƴ
괴NgAgo༭ľóۣʵ޷ظ
뺫˵ìܣȻ＼ӦҪ󺫹ԭʼݣü
Ȼûǰ;

תЭᴴߺԭϯLluis Montoliuʿһ
䱾ʵҵʵתЭԱһ⹫ţ
ֹͣͼ֤ʵ飬Ҫ˷ʱ䡢ǮԱĿ

CRISPRȸȺͺµĵ140ظУ
ֻһйشüã73شЧ63ش
֤ 

From: istt_list-bounces@transtechsociety.org on behalf of Lluis 
Montoliu To: ISTT List 
Subject: [ISTT_list] Great disappointment with Ago: Long life to 
CRISPR ! 
Date: Friday, July 29, 2016 9:49:22 AM 

Dear colleagues,  

the publication by Gao et al in May in Nature Biotechnology 
(http://www.ncbi.nlm.nih.gov/pubmed/27136078) triggered an enormous 
expectation. This Chinese team led by Chunyu Huan reported that the 
Argonaute (Ago) protein from a rare haloarchaea, Natronobacterium 
gregoryi, (NgAgo) would efficiently work for gene editing purposes in 
human cells. Ago had been described as an DNA-guided endonucleases two 
years before, through a Dutch-Spanish microbiologist collaborating 
team (Swarts et al. 2014, Nature: 
http://www.ncbi.nlm.nih.gov/pubmed/24531762).

On paper, the new (fourth) Gene Editing system looked great. An 
endonuclease, using ssDNA guides (5' phosphorylated though) and not 
RNA guides, without a PAM, requiring 24 nucleotides (and not 20nt), 
hence with higher specificity, and apparently with fewer off-target 
issues, since modifications in just one position of the DNA guide 
resulted in >90% decrease of the protein activity. 

On paper. 

I must confess we read the Huan paper in my lab with some 
disappointment, after two years battling, unsuccessfully, with Ago 
from Thermus, through a collaboration with my friend and colleague J. 
Berenguer, from the neighbouring reserch centre CBMSO, and one of the 
co-authors of the Nature 2014 paper. We had been scooped. We 
repeteadly failed to find any gene editing activity using Ago from 
Thermus thermophilus (TtAgo) in mammalian cells, through a variety of 
conditions and we didn't understand why, though we always suspected 
that these proteins would not be too comfortable at "too cold" 
temperatures as physiological +37C. After reading the Gao paper we 
concluded we simply missed the right bug and congratulated them for 
being smarter and lucky and for finding this archaea. Perhaps the 
trick was in using NgAgo instead of TtAgo.  

Shortly after NgAgo was released from Addgene, beginning of June, many 
labs, including mine, jumped onto it to try experiencing the 
anticipated great expectations and joy associated with this new tool 
of prokaryotic origin. But soon it was clear that something wasn't 
quite right. Rumours began spreading during June and July at congresses, 
through social networks, list emails and discussions groups that NgAgo 
didn't appear to work as reported. Actually, didn't work at all. Some 
colleagues that I absolutely trust at scientitic and technological 
levels started to indicate that they could not reproduce Huan's paper 
results. 

At the recent TAGC meeting (where IMGS was contributing to, merging in 
along with other Genetics Societies) Gaetan Burgio, from ANU, Camberra, 
Australia, presented some very preliminary data with a gel with some 
intermediate bands that would suggest NgAgo would be working and 
editing at the expected places. But, shortly thereafter, Gaetan 
engaged his lab in an OpenScience project, tried to characterize all 
these bands and.... found nothing. So, again, another evidence 
confirming NgAgo is not working as a gene-editing tool. 

Gaeatan just released today his experience using NgAgo, openly sharing 
his failures and providing details and some explanations for them. 

My experience with Natronobacterium gregoryi Argonaute (NgAgo) Gaetan 
Burgio Group leader at JCSMR, ANU 
https://medium.com/@GaetanBurgio/my-experience-with-natronobacterium-g
regoryi-argonaute-ngago-3ed8909b410c#.bo9y6mf9u  

At first, KUDOS to Gaetan. Many thanks to him for sharing their 
efforts trying to confirm some gene-editing activity associated with 
NgAgo. There is apparently none. In his view, NgAgo might be working 
as a ligase at physiological conditions. Similar to our negative 
results using TtAgo it would appear that NgAgo requires some higher 
temperatures to work as initially reported. This of course seeds some 
doubts on the Gao et al. publication and Gaetan, among other, is 
requesting to Nature Biotechnology to request the Huan's lab to reveal 
and share their raw data. We will see this part of the history how it 
develops...  

But, now, the most important message to convey is: NgAgo does not work 
for gene editing in mammalian cells. Be aware and do not waste your 
time, your money, your peoople and projects. If anyone has any 
positive hint suggesting Ago is indeed working as a genomic editor, 
please share the results, for the sake of Open Science, as Gaetan 
beautifully and most generously did. Many thanks to Gaetan!  

Unfortunately, this is a great disappointment. But, it also highlights 
the uniqueness and the robustness of the CRISPR-Cas systems. 

Long life to CRISPR!  

Lluis phone: +49621- 1703 6210/6204  

___________________________

Dr. Lluis Montoliu 
Investigador Cientifico - Research Scientist 
CSIC Centro Nacional de Biotecnologia (CNB-CSIC) 
Campus de Cantoblanco 
C/ Darwin, 3 
28049 Madrid (Spain) 
Tel. +34-91-5854844  / Fax +34-91-5854506 
e-mail: montoliu@cnb.csic.es 
WEB: http://www.cnb.csic.es/~montoliu/ 
U756 CIBERER: http://www.ciberer.es 
Spanish EMMA node: http://www.infrafrontier.eu 
ISTT: http://www.transtechsociety.org  

At present, I would recommend everyone abandoning any project 
involving the use of NgAgo. And avoid wasting time, money, animals and 
people. Results are clear in many labs. The results we have in Madrid 
are that TtAgo does not work in mammalian cells. The results that 
Gaetan has in Australia are that NgAgo does not work in mammalian cells. 
I know of many other colleagues who also tried and failed, but have 
not reported these failures publicly. This is why I posted this message. 
I think Ago might have some potential but we don't seem to have found 
yet the adequate version of it (or the right bug).

As a side history, Francis Mojica struggled to find Grants to support 
his pioneer experiments with CRISPR and got a couple of projects 
turned down. That is why, instead of using Streptococcus pyogenes 
(Doudna and Charpentier) or thermophilus (Siksnys), buga more 
difficult and expensive to grow, he chose to work in Escherichia coli 
CRISPR systems, and faced many difficulties, only to find out, many 
years later that E.coli CRISPR-Cas system was of type I (not type II, 
as Cas9) and, furthermore, was mostly inactive.

Please, let's focus in CRISPR-Cas9, Cpf1, C2c2,... et al... and leave 
alone Ago while microbiologists don't find out the right one.  

Lluis

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Աཨѧʵֻ1939ChernickĶ

ߣ



á

쿴ཨıԱƽѧ⣩ҵͬѧͬ
ܶ˶֪ཨ¼Ϊཨµķ̲֡סҪ˵. 
ϣܿǳô֪ô¡

ཨĽ1939ChernickĹϵĳ۵ĽҪܶ࣬ҲҪ
ܶࡣChernickУΪ˵2Ӧãȡr_1=1, r_2=2, r_3=3 
һӣǣ6k+1(12k+1)(18k+1)͵Carmichael
ڵǡö Chernickлr_1, r_2, r_3
ȡһЩֵõ͵Carmichael

Ȼ±ЦĻҲʹö2һЩĲôƯ
ôӣȡr_1=1, r_2=9k+2, r_3=3. Ӿ͵õ6k+1
(54k^2+12k+1)(18k+1) ͵CarmichaelཨġҪɹ
öһǸܴ˲ζͬķΧڣʽ
ֵĸˡ

ĳɹûκεļֵҪֻۣ˵
ǶԵġд2+2=4ۣҲֻ˵оǶԵġ

˵ཨĹʽ͹ϵĹʽЧʲôأֻ
֪ϵĴѣֻȽkֵûȥȽαֵһͬ
һΧڵģ6k+1(12k+1)(18k+1)͵Carmichael һͬһ
Χڵģ6k+1(54k^2+12k+1)(18k+1) ͵Carmichaelͻֹᷢϵ
6k+1(12k+1)(18k+1)ЧҪߵöࡣ

Chernick, J. (1939). "On Fermat's simple theorem" (PDF). Bull. Amer. 
Math. Soc. 45: 269C274. 

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ش

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ơƿǵҪעʵӦñͣ
ĳЩӲܲȫĲˣ͡ʳӦòȡ𽥼
ٵķΪθĽȴ̲˿ʼ

(XYS20160729)

˿(www.xys.org)(xys8.dxiong.com)(xys.ebookdiy.com)(fangzhouzi.me)

͢ת

ߣ Ȩ 
Դũҵ

726գһй߼ϣ͢ҵŵ
Mariano Lechardoy͢ʹũҵ޺նϣHernan Viola
͢ũҵũҵﰲȫƿƳάǣSilvia Passalacqua
ֱӦ˶ڰ͢תũҵʡũҵڴ¡

1͢ת
ʵǣ2015꣬͢2450תֲڵ
20142430ӣܵ͢ʳֲ3000ꡣ

2͢ũת
͢ũЩʹûκʣǻǳϧЩΪ
ʵʵ档

3͢νתģ
ŵת֮Ѿ֤䰲ȫԣҹҲ
ǵķɷϵԾͲҪٻˡ

һȻȻ飬תʳƷ۾һû
κε𣬼̣װˣȻͿˣȻҶҲû
ġڵĵڰ͢ûйð˵һ£
ǲڵĵЩأ

4͢бзתƷ
͢ĿǰûֲõıתƷӽеıƷ
һĴ󶹡͢ũԺһתϸԽֵС
ʹ߱õй̵á

5й˾͢תзĻߣ
ǶйҵǶκҵҵ͢
һġĿǰһйҵͰ͢ҵ˺˾ڹ
ͬתת壬Ҫڰ͢ҲҪȫΧۡ

6͢ԱתʳƷ
ŵұһٳתףҵĺҲԡ

7οתҥԣ
ûпѧݺоǰ¾תʳðȫĻк
صĺܲɸ˵ĿģҲĳһʹŻɡ

8ο˹ֲֹת
ʵһ߱ݣһֽףǿѧߡŷҲǻڴ˶
ųת

(XYS20160729)

˿(www.xys.org)(xys8.dxiong.com)(xys.ebookdiy.com)(fangzhouzi.me)

غϼ1252016.07.21-25


@fangshimin
TWEETS
10.6K
FOLLOWING
30
FOLLOWERS
83.4K

 ?@fangshimin  Jul 21 
תѸԴĻϿѧķչ
http://mp.weixin.qq.com/s?__biz=MjM5NTI3Nzc2Mw==&mid=2917739989&idx=1&sn=e24153fddd033025cad7351b3c4c9231&scene=0#wechat_redirect 

 ?@fangshimin  Jul 21 
¶Ľ
https://pbs.twimg.com/media/Cn7c1eAVYAApf6L.jpg
https://pbs.twimg.com/media/Cn7c1fXUsAAzQ7v.jpg
https://pbs.twimg.com/media/Cn7c1eOVMAAtv8Q.jpg
https://pbs.twimg.com/media/Cn7c1gOVIAEC99v.jpg

 ?@fangshimin  Jul 21 
żҿڡôҲɡʦˣǿɲǺôʡ@a109bc4355174d1 İ
ʦ

 ?@fangshimin  Jul 21 
սͳѡݽ屻й©Ȼעӣһһָͷ
ʵĻԡҪҪӦûǸֲ
ʵԵĲ̬˵ߡ
http://trumplies.com/an-advance-fact-check-of-trumps-remarks/ 
https://pbs.twimg.com/media/Cn7jY00VUAE4uuK.jpg

 ?@fangshimin  Jul 21 
˭ͳȴոδ֤֤Ǹ
̬Ͳ̬˵ߣѪͰºťˣƶƵһ
ȱݣʱ򻹲֪к@jsjrlhw Ϸϣﵱͳ
δ

 ?@fangshimin  Jul 21 
⻰ճй۲촨ʮ˸µĴԴĴ˵ģΪ
˽⴨գȻԴ˵ԴٽˮΪǵԴ
Ͷˮ@zhangjj13  ѪͰºť֪ڿ
ЦǺtrump޵ӽˮ

 ?@fangshimin  Jul 21 
֪ɵЦսǰڹۣȻ󱻵ȷըϡã
˵ӵ˷ٿǷ񷴻 || @LM19b: /z
Цͳը˭ը˭ͳҪսҲҪ׼Ȼ
ƹᣩսȨ޲ȳս

 ?@fangshimin  Jul 22 
ͳ´ִֻУܷԣͳΪ˾
@hiempty ûӿϿʼֻͳȨ´ʹú

Eddie Cheng ?@eddie__cheng  Jul 22 
ִֻвܷԡָǰ¡ϼͳ
µ¼ȨҲִܾСǰôսָŰͺը
ֲӼͥʱ漰⡣һӰ Crimson Tide Ҳġ 
@fangshimin @hiempty

 ?@fangshimin  Jul 22 
ΥԾִܾУ紨Ҫɱֲ޹˾
ԾܾҪ˵پ޴ΣҪú˵õ
鱨˼սҪȷˣô֪ǺϷǷǷ㲻ִ
ְһġ@eddie__cheng

elalemitbbs ?@elalemitbbs  Jul 21 
@fangshimin Homicides last year increased by 17% in Americas fifty 
largest cities. Thats the largest increase  in  25 yearsûбҥ
Ĳ

 ?@fangshimin  Jul 21 
ԭѾ˵ֻǺ˶һݣûг崨սлԡ
ѧ˲ŻΪûбҥ͵ģҲŻ᲻ƶø
˵¡@elalemitbbs

 ?@fangshimin  Jul 21 
նҫڷɱΣԱʹ
https://pbs.twimg.com/media/Cn7rc1WVIAAjDL0.jpg
https://pbs.twimg.com/media/Cn7rc1YVMAA3ho0.jpg

 ?@fangshimin  Jul 21 
˵ɱԱƬӦǱβ͵ȷǴġ

 ?@fangshimin  Jul 21 
ͱȻˡĿʣĶҪ¸Ұࣿ
@tsaifangfang ǿ.ûʲô.ܺ.ܿĿʣĶ.
ӵ.

 ?@fangshimin  Jul 21 
֮ΪܹѪʵһţΪˮɫѪ
Һ롣¡ܹѪ𣿡
http://fangzhouzi.baijia.baidu.com/article/551695  
http://xysblogs.org/fangshimin/archives/11544 

 ?@fangshimin  Jul 21 
ɶðͨΪԲͳ
˾ͳΨһȨսˣȷ
ͳϡܷľѱԭ䡣
https://pbs.twimg.com/media/Cn8Yp3OUIAAcs_o.jpg

 ?@fangshimin  Jul 21 
˰ȫרҷģͳʵӵ޵Ȩս
յͳĻûܹΥͳڽӵ˷
ľֻ6ʱǷ񷴻صͳʱһ󱨣
Һһ̽󱨡
http://www.politico.com/magazine/story/2016/06/2016-donald-trump-nuclear-weapons-missiles-nukes-button-launch-foreign-policy-213955

 ?@fangshimin  Jul 21 
άLaura Benantiģ´շݽ֪ĻΪǴշ˱ˡ
Ҫ浱ͳҪϲԱֻˡ
http://video.weibo.com/show?fid=1034:29b7935a630e4b93227394036b21c422 https://pbs.twimg.com/media/Cn80zd9VMAUxIyM.jpg

 ?@fangshimin  Jul 22 
ʯǴӲʯģʱ׿޻ûܼ
˳̣Ȼ25꣬26׿޽֡
https://pbs.twimg.com/media/Cn88eEvVUAAXFsx.jpg

 ?@fangshimin  Jul 22 
ͳҪҪսҪеѷľŰ͵Σ
ʱҪҪֻǿͳһ仰ʱľ¹ʶ鷢
һ̿ϾΪˣ˺ս
Ϊ侲ӢƢġսͳ

 ?@fangshimin  Jul 22 
úҪ׼Ǵ۶ķɣȻűȷˣ
@lonelytaste Ҫúг鱨֤˵ᣬǾͲ
˵ķΣ簲ȫ˵ֻΪķú䣬
볷α鱨ľٺָвĹᣬѶȲС

 ?@fangshimin  Jul 22 
ڹ͵ݽԴȫ
http://www.factcheck.org/2016/07/factchecking-trumps-big-speech/ 

 ?@fangshimin  Jul 22 
25ʣ½
https://pbs.twimg.com/media/CoBHnILUkAE_gPf.jpg

 ?@fangshimin  Jul 22 
˵˰صĹңʵ˰ڵ30λ
ŷޡ޸ɫСձ
https://pbs.twimg.com/media/CoBO-3VWAAA1Ig7.jpg

 ?@fangshimin  Jul 22 
ʦ
https://pbs.twimg.com/media/CoBW3vmWcAARGyR.jpg
https://pbs.twimg.com/media/CoBW7UhXEAA8mN1.jpg
https://pbs.twimg.com/media/CoBW3vwWYAELCsg.jpg
https://pbs.twimg.com/media/CoBW33_XEAAXetu.jpg

 ?@fangshimin  Jul 22 
ϣTim Kaine侺ѡ֮ǰѸKaineάĿ⼸
Ƶ޸ĲµˡΪһܶ˻ȥάҪǰ䡢޸
ϢάҪѡˡ
https://pbs.twimg.com/media/CoBeDXgWgAMJk-A.jpg

 ?@fangshimin  Jul 22 
ƱôԣŤŤåȫ֪åɷ
ֱˣ
https://pbs.twimg.com/media/CoBna0OVIAAl63G.jpg

 ?@fangshimin  Jul 22
ɽӺô
https://pbs.twimg.com/media/CoBwN2SVUAAwtIF.jpg
https://pbs.twimg.com/media/CoBwN2TUsAE2igP.jpg
https://pbs.twimg.com/media/CoBwN2ZUIAAWj3X.jpg
https://pbs.twimg.com/media/CoBwN-uUAAA_xnO.jpg

 ?@fangshimin  Jul 22 
йһԱҸԼʺӣ˭˵һҧ
ˣ˭ʣйһԱźС
һĶϰ
https://pbs.twimg.com/media/CoB7kNXVMAA3vGm.jpg

 ?@fangshimin  Jul 23 
׵١ɻСƼһµĹںšѧèͷӥ
ΪʲôҪԷרҵʿƼҽƷ
http://mp.weixin.qq.com/s?__biz=MzIxMTQ5NjkzNA==&mid=2247483664&idx=1&sn=dea25f7a39534fdecd8944d04ab20387&scene=2&srcid=0723r9jEXcLQewZujykUuGX7 

 ?@fangshimin  Jul 23 
ȫɽһƬ죬Ҳﵽ26϶ȡ
https://pbs.twimg.com/media/CoF1ZNIVUAAhedU.jpg
https://pbs.twimg.com/media/CoF1ZNJUEAAoWHu.jpg

 ?@fangshimin  Jul 23

https://pbs.twimg.com/media/CoF9Q6fUkAA_WUY.jpg
https://pbs.twimg.com/media/CoF9Q6jUsAE8JO2.jpg
https://pbs.twimg.com/media/CoF9Q6qVIAI8mpU.jpg
https://pbs.twimg.com/media/CoF9RANVYAA5AbE.jpg

Eddie Cheng ?@eddie__cheng  Jul 23 
ǰѡ˿John KerryҲͽⷽѺгˣ
Ρ
https://pbs.twimg.com/media/CoGAuoZUMAA7gM3.jpg

 ?@fangshimin  Jul 23 
ķ.Ϊͽ˷Զ̥ǿָ֧ŮѡȨΪ
Ƿ̥ӦɸŮԼӦԤŮֳ@eddie__cheng 
ǰѡ˿John KerryҲͽⷽѺгˣ
Ρ

 ?@fangshimin  Jul 23 

https://pbs.twimg.com/media/CoGIMmYUMAAduRl.jpg

 ?@fangshimin  Jul 23
ʨ
https://pbs.twimg.com/media/CoGQ72WVIAE8hBQ.jpg
https://pbs.twimg.com/media/CoGQ72ZVMAAfRKg.jpg
https://pbs.twimg.com/media/CoGQ72iUMAIxsqv.jpg

 ?@fangshimin  Jul 23 
ֲĿ־壬ߡɿ߼׷Ҳÿ־̤
ɡ
https://pbs.twimg.com/media/CoGYDC4UEAAyGQ9.jpg

 ?@zixian  Jul 23
 Retweeted Olivia Deng
Well, they came up naturally... added,
Olivia Deng ?@oliviadeng1
My dad has an Instagram now and the first accounts he follows - 
friendly.atheist, americanatheists

 ?@fangshimin  Jul 23
Naturally born atheist. @zixian Well, they came up naturally...

 ?@fangshimin  Jul 23 
ӡǸѧ˵ûظˣƪЩʱŵ 
ʵظɹ
http://hebei.ifeng.com/a/20160724/4792091_0.shtml 

 ?@fangshimin  Jul 24 
ѧУȰѼǧʽ㵽˵@MarvinGPP0  ѧУҲҪ
λӦ ֮ǰ˵Ͷʷ˵ظʵؼ100ڵ¹
ֱѧУ˵ˡɶ

 ?@fangshimin  Jul 23 
ƭ԰ĿƭΪץסʲôìܣˡ̵Ȼ
ˡظóЧʵָͣиЧʣ롱ظóС¼ָ
ظʣߵģȻֻȥͱھ񲡵ĴȡȰ
Զƭ԰
https://pbs.twimg.com/media/CoGpSTfUMAAsELu.jpg
https://pbs.twimg.com/media/CoGpSTcVUAAlo90.jpg
https://pbs.twimg.com/media/CoGpSXBVYAA7dsa.jpg
https://pbs.twimg.com/media/CoGpSa3UEAAB_CF.jpg

 ?@fangshimin  Jul 24 
ٰΪΣҪôпƭ@1ʺЧʵ
ҲˣЦǿƭ԰ƭκȻҲ֮һҶִˣ
Ҫڽ¶ѧ٣ƭ԰˼ѧȴҥĨΧ
ӣһһͣ֡ûȰԶƭ԰

 ?@fangshimin  Jul 24 
Ѷ㵽Ļļƭ԰㳲ֻͼƬ
@hqabc һ٣ŶӰ顣

 ?@fangshimin  Jul 23 
ʢʱִյĳƪֽۡͨôһͳ
ѡˣǸⰸ˵ΪһͣɢԵϰ
˵ߣ֪ԼѻԵʵ֪ǻԵںҲ֪
ˣ
https://www.washingtonpost.com/opinions/donald-trump-is-a-unique-threat-to-american-democracy/2016/07/22/a6d823cc-4f4f-11e6-aa14-e0c1087f7583_story.html

 ?@fangshimin  Jul 24 
ܶ໪ԼߣΪ͡бҲ
ԼѣֻԱѺܷŭ@Zhongxiling ʦҺܲһ
ǣĻ˺öִ֧հһЩܹʿͬѧô
ԵĻ©ӣΪʲôôͦأ

 ?@fangshimin  Jul 23 
շ԰°ˣҹ
https://pbs.twimg.com/media/CoG7RLYVUAAlSTn.jpg
https://pbs.twimg.com/media/CoG7RM6UAAAzARV.jpg
https://pbs.twimg.com/media/CoG7RQoUkAA-8wq.jpg
https://pbs.twimg.com/media/CoG7RSIUMAAT6nO.jpg

 ?@fangshimin  Jul 23 
ĽڴǹɱǸѧУ۸ʮ¹ˣͨα
ҳѳԷܺߣܺ߰3С3С1
ϣСɶΡڽ̶¼ΣЩ
̬ôҲ
https://pbs.twimg.com/media/CoHB3s6UMAAwdTW.jpg

 ?@fangshimin  Jul 24 
ҪʲôˮӲŻšȫٷ֮ʮϵĿֲϮ˹ָ
ġҥԡҲǰѰʲôĶ˹ˡ
@yangyueke ȫٷ֮ʮϵĿֲϮ˹ָɵģ˹û
ϵ

 ?@fangshimin  Jul 24 
ֻһΰ󡱣޷֤ʵԵ¹
ˣɱҲΪ˵Ķǳ
֪о񲡣ҽ@Bovyli Ϯ߱߿ǹ߸ߺǵ¹
ˣAllahu Akbarɶڽ̶

look1m ?@look1m  Jul 23 
ĿңȻҪĽܵһСֲ
λûҪӢԴա˵СˣЩ˵ת˿֮
ӡӷȺáĻǡ⼸λΪֻΪ˵߼
ǺڷΪʲô棿
https://pbs.twimg.com/media/CoFlPi3UEAQim9C.jpg

 ?@fangshimin  Jul 23 
ƭϣҵ˫ң@look1m ĿңȻҪĽ
ܵһСֲλûҪӢԴա˵
СˣЩ˵ת˿֮ӡӷȺáĻǡ
⼸λΪֻΪ˵߼ǺڷΪʲô棿

 ?@fangshimin  Jul 24
ƽ
https://pbs.twimg.com/media/CoK8UseVMAAbriV.jpg

 ?@fangshimin  Jul 24 
߲ҧˣֻ󣬽Ǳ£ҪзԵĿ־塣
@maijian338 Ƕʲôأһŭô졣

 ?@fangshimin  Jul 24 

https://pbs.twimg.com/media/CoLCLjpVYAE1DtC.jpg
https://pbs.twimg.com/media/CoLCLjsUIAAh2I4.jpg

 ?@fangshimin  Jul 24 

https://pbs.twimg.com/media/CoLIz25VUAEQsxR.jpg
https://pbs.twimg.com/media/CoLIz3DVYAAaZG1.jpg
https://pbs.twimg.com/media/CoLIz3EUkAA-72d.jpg

 ?@fangshimin  Jul 24 
FMĿӽѧ73Ϊʲôе˳ˮӣ
http://fm.qq.com/luobo/radio?_wv=4097&showid=rd00400kYL0G7ts5&aid=rd00
1USq1949X1mp&from=groupmessage&isappinstalled=1 

 ?@fangshimin  Jul 24 
ŷ޵ĿֲϮ2%˹ָɵġʲôˮӲŻ
90%ϵĿֲϮ˹ָɵҥԡ
http://thinkprogress.org/world/2015/01/08/3609796/islamist-terrorism-europe/ 
https://pbs.twimg.com/media/CoLb_JuXgAE88d2.jpg

 ?@fangshimin  Jul 24 
ԭʲôǿֲϮѡֲϮɡԼ
ֲϮͿԼҥˡ@ericxu1997 ҪֲϮ
ֲϮ˹ռڽİ
˵ɱֲϮ˵98%˹ָɵĶеˡ

 ?@fangshimin  Jul 24 
ϲʲ˵ôŮͶƱգ̨ձĵ
Ůʲô˾֪ˣһҽҹ˽봨۵ı
ۿ۵ȷҽһ˼άңҸоá
https://pbs.twimg.com/media/CoLizmeXYAANMwz.jpg
https://pbs.twimg.com/media/CoLiznOWcAAz7lo.jpg

 ?@fangshimin  Jul 24 
ΪЩϸĵطʧЧƶģЭĽ
ΪڿɣûдﵽЩתʿҪʵ൱
ġ¡ΪʲôҪʶתʳƷ
http://fangzhouzi.baijia.baidu.com/article/554903 

 ?@fangshimin  Jul 24 
ŷ޾֯·ı棬2015ŷ޹211ʧܻɹĿֲ
Ϯ65Ƿ߸ɵģ17˹ʥսӸɵģ࣬
2014ֻ4𣩣13֯ɵģ9Ǽ֯ɵġ
https://www.europol.europa.eu/sites/default/files/publications/europol_tesat_2016.pdf 

 ?@fangshimin  Jul 25 
ҷǷ̬Ƶ99%ֲϮ¼˹ָɵҥԣŷ
2015ΪҲֻռ10%̬ҪĿ̸ˣ2015һ꣬
ΪаҰ20142013̬ǲҪԴˣ
@durgeeda

 ?@fangshimin  Jul 24 
ֲ˱̨ͬĳԻߵĳһµġ
https://pbs.twimg.com/media/CoMKECLUsAA5IO9.jpg

 ?@fangshimin  Jul 24 
ܶй˳ݵľִţƤġǿˡգû˭Ҹ˽
ϵͳһ˾޺
https://pbs.twimg.com/media/CoMRtfpVUAA7MNc.jpg

 ?@fangshimin  Jul 25 
ѧУbullyСеġֻЩ
˷ϲbully@MolecularCell15 ҸҶӣ7꣩һδսܲ
õƵһ룬Ҷ˵ҪѡѡգΪ˵󻰡
ôģ

 ?@fangshimin  Jul 25 
ǿʯͷģ׿ûйϵ׿޳ǰͱ
ѡɱĵ̼ҵ̴׿ֻǺ;֡ҥϴ
̸ǫǻͽġġ
https://pbs.twimg.com/media/CoMcbK0UIAAwdkH.jpg

 ?@fangshimin  Jul 25 
ѧʿʿѧλĳϮʿѧλǽ¶ѧ
ٵһССĳɹ
https://pbs.twimg.com/media/CoMqkEcUEAE-wnZ.jpg

 ?@fangshimin  Jul 25 
Ƚȷе֤ݱȾܵ¿ʰʳΰ᳦ֱ
ְٰ֢ܵ±İ֢緶Χڰ֢Լ6%ǺȾƵ
µġ
http://onlinelibrary.wiley.com/doi/10.1111/add.13477/abstract;jsessionid=E44F4E532B0A1F7E0DFFE4F435CED53F.f03t02 
https://pbs.twimg.com/media/CoQLX6ZVUAE-tLY.jpg

 ?@fangshimin  Jul 25 
ջԵس̶ȶԶԶϣǴ֧߶Դں
ϣĻԣееģΪŭ˸
˼˳֮Ϊҳϡ
https://pbs.twimg.com/media/CoQSZTkVMAAb-c5.jpg
https://pbs.twimg.com/media/CoQSZTnUAAAbVM6.jpg

Eddie Cheng ?@eddie__cheng  Jul 25 
ڹһϣڹ֤α֤ûбصˡ 
@fangshimin

 Junbiao Han ?@JunbiaoH  Jul 25 
@eddie__cheng @fangshimin Ϊ֮ǰûģΪ˵
թΪлߣԼbragְҵְҵ˭ĳ̶ȸ
ܶ˵ĶȡᣬΪ̿ԤƵĸ⣬ȴԽԽ
ֳΪѵһľˡ

 Eddie Cheng ?@eddie__cheng  Jul 25 
ǡԽԽԴӴճǰμӵѡٶ
Ȩȡᡱδһǰ˵Ϊٵв
սͿֲϮȷΨһһȷ濹
ѡˡ @JunbiaoH @fangshimin

MarvinGPP ?@MarvinGPP0  Jul 25 
@eddie__cheng @JunbiaoH @fangshimin ȷȻǽսв
˵˵ȷôвˣôвˣ

 Eddie Cheng ?@eddie__cheng  Jul 26 
Ϊȷܶ˱ܾУݽڲҿĳЩΣǵ
⵽вҲǰ⵽вΪȷǷ
áǷ֣вı߾ȫͷϡ̫ˣ޷о١ 
@MarvinGPP0 @JunbiaoH @fangshimin

 ?@fangshimin  Jul 26 
ûβȷġɡôʴʻûץأ
ΪȺܵǲûɡ
@eddie__cheng

 Eddie Cheng ?@eddie__cheng  Jul 25 
δѡܿܳΪýΪ䣨߰ררõȵȣ
ѡΪӢ¢˷ҡ @zhangjj13 
@JunbiaoH @fangshimin

 ?@fangshimin  Jul 25 
ʱýԽԽССʲôܵѡΣѿ
µļҵ̣ꣿСʲֻ͡˵ҪǴյѡ
Ҫöࡣ@eddie__cheng

 ?@fangshimin  Jul 26 
νɿ߾ģԭ@JunbiaoH ڽܲɷõʱ򣬵
˵һϸڽߵʱȻϲǿԼô
religious˵ȫǷȷֻǷӭڶѡһ
ִ󲿷ֵ˶˹֣Ҳްġ

 ?@fangshimin  Jul 25 
յĿںǡٴΰ󡱣ôصĸʱ80
սвս70Խսˮ¼60ϡ𱻴ɱ
Ȩ˶50壻40ս30203K
10һսҲصֶȨ90

 ?@fangshimin  Jul 25 
ĿǰԶٵ̴ӶߵĽգԲ֪ôû
һ׼ֻܶԵ˵ܵؼ뵨̴¡̴
ƽ𣿡http://fangzhouzi.baijia.baidu.com/article/557197  
http://xysblogs.org/fangshimin/archives/11550 

 ?@fangshimin  Jul 25 
ҹַܻǹʮС17ˡ˹
ֻǻͽɵİ
https://pbs.twimg.com/media/CoQy6xVUEAEJJFp.jpg

 ?@fangshimin  Jul 25 
ϣҪûϹѧİѡ񡣺2008°ѡȣ
֧Ⱥûɶ仯ûϴѧİѡ20㡣ƴշ
ϣҲΪ˹Ⱥһ66%ִ֧գ29%֧
ϣ
https://pbs.twimg.com/media/CoQ3wIDVIAADJ6D.jpg

 ?@fangshimin  Jul 25 
ͳǷѡģԣݣѡѡˣ
ƶݣϷвѡ͵ѡˣ˭ѡǼ
ҡݾȫֻ⼸ҡ׼ȷ
https://pbs.twimg.com/media/CoQ_3YQUEAINTjt.jpg

 ?@fangshimin  Jul 25 
֪ʶӡϺƽһѳĻоƷѵҲ
ǴӱƷ˾õ
https://pbs.twimg.com/media/CoRPuvLVUAEz02i.jpg

 ?@fangshimin  Jul 25 
Ǵ󲿷֧ġPaul Simonϲһ׸衶ˮϵ
šǰĺá@eddie__cheng ȹ͵ǿ
Ƕࡢߣݳʵö࣬һ

Eddie Cheng ?@eddie__cheng  Jul 25 
ε¼ֵǺ֪ơļ
ѧˡ²дյʲôڲϣһֱûвȡƻ
κӦʩȫ֪ΪĴһĵ죬û
ЩС

 ?@fangshimin  Jul 26 
ûسɶϡȫίԱƫϣֲʲôܣ֪Ϊι͵Ҳ
¹ϣ񹲺͵ȫίԱͶɾƵġ@eddie__cheng

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